Advances in Horticultural Science <p><strong><em>Advances in Horticultural Science&nbsp;</em></strong>aims to provide a forum for original investigations in horticulture, viticulture and oliviculture. The journal publishes fully refereed papers which cover applied and theoretical approaches to the most recent studies of all areas of horticulture - fruit growing, vegetable growing, viticulture, floriculture, medicinal plants, ornamental gardening, garden and landscape architecture, in temperate, subtropical and tropical regions. Papers on horticultural aspects of agronomic, breeding, biotechnology, entomology, irrigation and plant stress physiology, plant nutrition, plant protection, plant pathology, and pre and post harvest physiology, are also welcomed.</p> <p>The journal scope is the promotion of a sustainable increase of the quantity and quality of horticultural products and the transfer of the new knowledge in the field.</p> <p>Papers should report original research, should be methodologically sound and of relevance to the international scientific community.</p> <p>AHS publishes three types of manuscripts: Full-length - short note - review papers. Papers are published in English.</p> Firenze University Press en-US Advances in Horticultural Science 0394-6169 <p>Authors retain the copyright and grant the journal right of first publication with the work simultaneously licensed under a <strong>Creative Commons Attribution 4.0 International Public License (<a href="">CC-BY-4.0</a>)</strong>&nbsp;that allows others to share the work with an acknowledgment of the work's authorship and initial publication.</p> <p><a href="" rel="license"><img src="" alt="Creative Commons License"></a><br>This work is licensed under a <a href="" rel="license">Creative Commons Attribution 4.0 International License</a></p> Secondary metabolite changes in Maymars juniper cuttings (Juniperus sabina) under different treatments of propagation (IBA, substrate and harvest time of cutting) <p><strong>The Endemic Juniper of Maymars (<em>Juniperus sabina</em>) is one of the most valuable plants in forested areas. The objectives of this experiment were: I) to determine the best conditions for stem cutting propagation of this species, and II) to examine changes in some of the secondary metabolites during the four months (the first of each season): January, April, July, and October, after rooting of cuttings. The research was done with the treatment of five levels of Indole Butyric Acid, including: 0, 1000, 2000, 4000, and 8000 ppm in four rooting substrates, including perlite, perlite-cocopeat (1:1), pumice, and a mixed rooting substrate (sand, perlite, cocopeat, vermicompost, and potash; 1:1:1:1:1) in the four seasons of the year, with stem cuttings having an average length of 15 cm. The best treatment with more than 50% rooting was seen in April at levels of 4000 and 1000 ppm, and the best substrate was perlite cocopeat. Using lower levels of IBA led to a reduction in total phenol content in the cuttings during the rooting period. The flavonoid content of the cuttings varied across different seasons. Based on these results, we recommend this way of propagation for <em>Juniperus sabina</em> production. This propagation method takes less time in comparison with sexual propagation from seed.</strong></p> Maliheh Abshahi Hossein Zarei Bahman Zahedi Francisco Antonio García-Morote Abdolhossein Rezaei Nejad Copyright (c) 2022 Maliheh Abshahi 2022-05-05 2022-05-05 36 3 163 174 10.36253/ahsc-13044 Physicochemical characteristic and internal browning of pineapple as affected by calcium and gibberellic acid dipping application <p><strong>postharvest applications of Calcium and gibberellic acid have proved to maintain optimal fruit quality and control decay during cold storage. This study evaluated the effect of calcium and gibberellic acid dipping application on pineapple quality and internal browning. The experiment implemented two factors. The first factor concerning two dipping times (five and ten minutes) and the second factor related to </strong><strong>four treatments, A </strong><strong>(gibberellic acid), B (calcium), C (calcium + gibberellic), and control (D) - (no calcium or gibberellic acid applied). Total soluble solids (TSS), total acidity (TA), TSS/TA ratio, sugar, citric and ascorbic acid content, together with browning severity and incidence, were determined. Treatment B, essentially using a dipping time of five minutes, delivered the best performance having the lowest severity and incidence of browning (4.44 and 22.22 %, respectively), the highest citric acid (0.61 %), ascorbic acid content (405.18 mg kg<sup>-1</sup>) and TSS/TA ratio (25.53). Meanwhile, the other treatments were considered less satisfactory, due to their highest browning severity and incidence, without remarkable impact on the citric acid and ascorbic acid content, especially with a dipping time of ten minutes. In conclusion, dipping applications of calcium and gibberellic in postharvest enhanced pineapple quality and reduced the internal browning. </strong></p> Diego Mauricio Cano Reinoso Rafi Renaldy Tamalea Condro Wibowo Copyright (c) 2022 Diego Mauricio Cano Reinoso, Rafi Renaldy Tamalea , Condro Wibowo 2022-06-06 2022-06-06 36 3 175 184 10.36253/ahsc-12535 Impact of cultural conditions on germination of olive (Olea europaea L.) somatic embryos and plantlets development from the Algerian cultivar Chemlal <p><strong>The <em>in vitro</em> propagation techniques are currently a commercial alternative for the production of plants with good quality in several plant species, including the olive tree (<em>Olea europaea</em> L.). Somatic embryogenesis is the process practically used for the application of several biotechnological tools of improvement and <em>in vitro </em>plant regeneration via the germination of somatic embryos. Our work aims to evaluate the effect of the chemical and hormonal composition of the culture medium on the germination of olive somatic embryos (cv. Chemlal) as well as the micropropagation of the obtained plantlets before their acclimatization to natural conditions. The results indicated that the production of olive plants by somatic embryogenesis depends strongly on the genotype of the somatic embryos (cell line) and more on the culture conditions, particularly the presence of growth regulators. Indeed, a solid OM medium supplemented with hormones (BA and IBA) permitted an advanced root emergence and germination allowing the production of well-developed plants with several leaves. In addition, an OM medium supplemented with Zeatin and IBA allowed better reactivity of micro-cuttings producing well-developed shoots with several emitted roots which facilitates their further acclimatization to natural conditions.</strong></p> Khayreddine Titouh Khadidja Hadj Moussa Nazim Boufis Lakhdar Khelifi Copyright (c) 2022 Khayreddine Titouh, Nazim Boufis, Khadidja Hadj Moussa, Lakhdar Khelifi 2022-06-23 2022-06-23 36 3 185 191 10.36253/ahsc-12488 Maintaining postharvest quality of bell pepper (Capsicum annuum L. cv. California Wonder) using cactus (Opuntia stricta L.) mucilage coating <p><strong>Bell pepper (<em>Capsicum annuum</em> L.) experiences significant qualitative and quantitative loss during postharvest. This study aimed at providing an alternative postharvest handling technology for bell pepper. The factor studied was cactus (<em>Opuntia stricta</em> L.) mucilage coating at four levels: 0% (distilled water), 1, 2, and 3%. The fruits were stored under ambient conditions (25 ± 2°C temperature and 65 ± 2% relative humidity) until senescence. Weight loss and total soluble solids content were determined at an interval of 3 days whereas iron and ascorbic acid content were determined at an interval of 4 days. Shelf life elapsed when fruit lost 25% of their initial weight on average. Cactus mucilage coating reduced weight loss by up to 21.64%, maintained total soluble solids by up to 14.93%, iron by up to 6.46%, ascorbic acid by up to 19.46% and extended shelf life by up to 6 days. Cactus mucilage coating at 1% was the best treatment and therefore can be used by bell pepper growers, retailers, and consumers to maintain postharvest quality and extend shelf life of bell pepper.</strong></p> Maurine Aloo Arnold Mathew Opiyo Mwanarusi Saidi Copyright (c) 2022 Maurine Aloo, Arnold Mathew Opiyo, Mwanarusi Saidi 2022-07-05 2022-07-05 36 3 193 200 10.36253/ahsc-12275 Organic amendments role in reducing drought stress in Alcea Rosea L. <p><strong>Water scarcity and dwindling natural resources due to global warming are negatively impacting ornamental plant survival. Soil fertility remains a problem in arid and semiarid regions. In this study, the effects of four media (arable soil, arable soil + cow manure, arable soil + rice hull, arable soil + wheat straw) on macronutrient content and quantitative characteristics of Alcea rosea L. under drought stress were investigated. Application of organic amendments mitigated the negative effects of drought in the soil and increased the available organic macronutrients. The application of organic amendments increased the total N, P, and K content in the soil and leaves of hollyhock. Total soluble sugars (by 11.9%), RWC (by 8.75%) and phenolics (by 36.4%) of hollyhock were significantly improved by the application of organic amendments at 80% FC. The amended soil (soil + cow manure) increased the activities of superoxide dismutase and ascorbate peroxidase at 80% FC. Moreover, the soil + cow manure proved to be the best supplement to improve leaf area and dry weight. In conclusion, the application of organic amendments can be successfully used as a cost-effective management method to improve soil fertility and crop production in arid and semi-arid areas.</strong></p> Atiyeh Oraee Mahmoud Shoor Toktam Oraee Ali Tehranifar Hossein Nemati Copyright (c) 2022 Atiyeh Oraee, Mahmoud Shoor, Toktam Oraee, Ali Tehranifar, Hossein Nemati 2022-07-29 2022-07-29 36 3 201 214 10.36253/ahsc-12185 Genetic and ampelographic characterization of grapevine accessions maintained in the Lebanese national collection <p><strong>Safeguarding grapevine biodiversity is one of the main concerns in viticulture today. Management of <em>ex situ</em> collections requires a comprehensive characterization of the conserved germplasm to provide basic material for selection and breeding programs. In this study, the local grapevine germplasm conserved at the national collection of the Lebanese Agricultural Research Institute and composed of 43 accessions was submitted to a genetic and ampelographic study. Nine ISSR primers, previously developed in grapevine, were used in this study. These primers generated a total of 51 bands, of which 77.7% were polymorphic allowing the differentiation of 41 genetic profiles <em>vs.</em> one case of synonymy that was recorded among three accessions. Ampelographic characterization was conducted using a set of 33 descriptors established by the International Office of Vine and Wine and related to leaf, bunch, and berry. Principal component analysis identified 12 descriptors i.e. v</strong><strong>eraison date, maturity date, berry length, upper and lower vein pigmentation, bunch density, bunch weight, sugar content at harvesting, flesh of juiciness, berry weight, flesh firmness, and color skin, as being the most discriminating descriptors. </strong><strong>The correlation between the ISSR clustering and the ampelographic one was not significant (r=0.26) because of the divergence of accessions groups, except for the three accessions synonymy case which was confirmed in both dendrograms. Finally, this comprehensive evaluation of the existing local gene pool of grapevine revealed a substantial diversity. It would further allow the promotion of the valuable accessions directly through multiplication schemes, and their sustainable utilization in genetic improvement programs. </strong></p> Ali Chehade Lamis Chalak Joe Merheb Ahmad Elbitar Elie Rmeily Nagham Madi Mark Massaad Copyright (c) 2022 Ali Chehade, Lamis Chalak, Joe Merheb; Elie Rmeily; Ahmad ElBitar, Nagham Madi, Mark Massaad 2022-08-24 2022-08-24 36 3 215 226 10.36253/ahsc-13045 Economic viability and development of radish (Raphanus sativus L.) under different soil water tensions and mulching types <p><strong>There is a lack of information on the production of irrigated radish associated with the use of mulching and on the economic viability of these production technologies. The objective of this study was to evaluate the growth, yield, and economic viability of the radish crop under different soil water tensions (SWT) and mulching types. The experiment was conducted in a greenhouse. During the experiment, the following variables were evaluates: growth parameters, yield and economic viability. SWT at 7 kpa in the treatments without mulching and at 12 kPa in the treatments with black plastic and black non-woven resulted in higher growth parameters and yield. The leaf area index and the root diameter were the parameters that had a high and positive correlation with yield. Expenses with variable resources represent on average 75% of the total production cost. Therefore, the investment pays all resources applied in the activity and provides an economic profit. In this context, the higher radish yield with 37.5 t ha<sup>-1</sup> provided the highest profitability of the evaluated treatments, thus, for radish production, the recommendation is to use 12 kPa as an indicator of the moment for irrigation, associated with the use of black plastic.</strong></p> Pietros André Balbino dos Santos Luiz Gonsaga de Carvalho Felipe Schwerz Victor Buono da Silva Batista Cassio Augusto Ussi Monti Copyright (c) 2022 Pietros André Balbino dos Santos, Luiz Gonsaga de Carvalho, Felipe Schwerz, Victor Buono da Silva Batista, Cassio Augusto Ussi Monti 2022-08-24 2022-08-24 36 3 227 237 10.36253/ahsc-12552 Contrasting aspects of the physical and physiological dormancy shown by seeds from different peach rootstocks <p><strong>Considering that cultivars of peach rootstocks selected to be propagated by seeds show variation in the degree of physiological dormancy and peculiarities regarding the limitation imposed by the endocarp, it is essential to define the cold stratification period and the ideal temperature to be used during the pre-germination treatment. Isolated for each cultivar, knowledge of these variables which will provide viability in the production of peach rootstocks via seeds, in the presence of the endocarp, at a larger scale. Two germination tests were carried out, in a completely randomized experimental design and 4 x 3 factorial scheme, with four replications, each one consisting of 25 endocarps. The first experiment was performed with four cultivars (Aldrighi, Capdebosq, Okinawa Roxo and Tsukuba 1) and three stratification temperatures (1°C, 4°C and 7°C) for a period of 90 days. For the second experiment, the same cultivars were used, two temperatures (7°C for ‘Aldrighi’ and ‘Capdebosq’ and 4°C for ‘Okinawa Roxo’ and ‘Tsukuba’) and three stratification periods (40, 80 and 120 days), followed by sowing in substrate under greenhouse conditions for a period of 45 days, with subsequent endocarp breaking and re-sowing. The pre-germination treatment at 7°C for 90 days is sufficient to obtain high germination percentage of ‘Aldrighi’ and ‘Capdeboscq’ seeds in the presence of the endocarp. Under the stratification conditions tested, the seeds of ‘Okinawa Roxo’ and ‘Tsukuba 1’ require rupture of the endocarp to reactivate the germinative embryonic process.</strong></p> Renata Diana Menegatti Marcos Aurélio C. Lima Aline G. Souza Oscar José Smiderle Valmor João Bianchi Copyright (c) 2022 Renata Diana Menegatti, Marcos Aurélio C. de Lima, Aline das Graças Souza, Oscar José Smiderle, Valmor João Bianchi 2022-07-29 2022-07-29 36 3 239 245 10.36253/ahsc-12192 Evaluation of Hemerocallis germplasm using single nucleotide polymorphisms of nrITS and chloroplast interspacer region <p><strong>This study was initiated to distinguish nocturnal (night) flowering <em>Hemerocallis</em> species from day flowering species based on the single nucleotide polymorphisms (SNPs) of nuclear internal transcribed spacers 1, 2 in a ribosomal RNA gene (nrITS) and a chloroplast interspacer region (cpIS). Four nocturnal flowering species, <em>H. &nbsp;citrina</em>, <em>H. thunbergii</em>, <em>H. minor</em>, and <em>H. lilioasphodelus,</em> were collected including Korea, and compared with day flowering species that included <em>H. vespertina</em> and <em>H. hongdoensis.</em> Based on the haplotypes of nrITS and cpIS, nocturnal species cannot be distinguished from day flowering species. Discrepancies in flowering time and haplotypes among <em>H. minor</em> accessions suggest that more germplasm with diverse geographic origins should be evaluated and identification of other genes is required to effectively distinguish nocturnal species from day flowering species.</strong></p> Seo Young Park Young Hee Joung Jeung Keun Suh Mark Roh Copyright (c) 2022 Mark Roh, Seo Young Park, Young Hee Joung, Jeung Keun Suh 2022-10-04 2022-10-04 36 3 247 251