New record and dietary ecology of a poorly known amphibian species, Micryletta menglienica (Yang and Su, 1980), from Vietnam
Published 2025-05-06
Keywords
- Formicidae,
- Prey items,
- Rhinotermitidae,
- Son La Province,
- Stomach contents
How to Cite
Copyright (c) 2025 Hai Ngoc Ngo, Anh Van Pham, Truong Nguyen, Nenh Ba Sung, Thuy Van Tran, Minh Duc Le, Anh Ngoc Thi Ho

This work is licensed under a Creative Commons Attribution 4.0 International License.
Abstract
Recent field surveys revealed a new population of the Menglian Paddy Frog (Micryletta menglienica) from Son La Province, northwestern Vietnam based on molecular and morphological analyses. The species has been listed as “Data Deficient” in the IUCN Red List. To date, knowledge about the natural history of this species is scarce, including data on its dietary ecology. Using the stomach-flushing method, we analyzed stomach contents of 45 individuals (21 males and 24 females) from a total of 52 captured frogs because stomachs of six males and one female (13.5%) were empty. The food spectrum of M. menglienica comprises 12 types of 691 prey items (687 invertebrate items and four unidentified items), belonging to six insect orders, larvae and Araneae. Similar to other species of the family Microhylidae, M. menglienica is also identified as an ant (Formicidae) – termite (Rhinotermitidae) eating specialist with distinctly higher percentages of occurrence frequency (F = 65 %), number of prey items (N = 80 %), and importance (Ix = 75 %) than those of remaining types. The Simpson diversity index of 0.939 shows a high abundance of food prey in the wild, and the Evenness index of 0.536 suggests biased consumption of feeding selection for M. menglienica.
References
- Atencia, P., Conde, Ó., Solano, L. (2017): Use of biological resources by Elachistocleis pearsei (Ruthven, 1914) (Anura: Microhylidae) in a lentic ecosystem from the department of Sucre, Colombia. Acta Zool. Mex. 33: 130-132.
- Berazategui, M., Camargo, A., Maneyro, R. (2007): Environmental and seasonal variation in the diet of Elachistocleis bicolor (Guérin-Méneville 1838) (Anura: Microhylidae) from northern Uruguay. Zool. Sci. 24: 225-231.
- Burton, T.M., Likens, G.E. (1975): Energy flow and nutrient cycling in salamander populations in the Hubbard Brook Experimental Forest, New Hampshire. Ecology. 56: 1068-1080.
- Caldart, V.M., Lop, S., Bertaso, T.R.N., Zanini, C. (2012): Feeding ecology of Crossodactylus schmidti (Anura: Hylodidae) in southern Brazil. Zool. Stud. 51: 484-493.
- Clavel, J., Julliard, R., Devictor, V. (2011): Worldwide decline of specialist species: toward a global functional homogenization? Front. Ecol. Environ. 9: 222-228.
- Clements, R., Sodhi, N. S., Schilthuizen, M., Ng, P. K. (2006): Limestone karsts of Southeast Asia: imperiled arks of biodiversity. Bioscience. 56: 733-742
- Duellman, W.E., Trueb, L. (1994): Biology of Amphibians. The John Hopkins University Press, Baltimore, USA.
- Duré, M.I., Kehr, A.I., Schaefer, E.F. (2009): Niche overlap and resource partitioning among five sympatric bufonids (Anura, Bufonidae) from northeastern Argentina. J. Herpetol. 8: 27-39.
- Fei, L. (2020): Atlas of Amphibians in China (Field Edition). Zhengzhou, Henan science and Technology Press.
- Frost, Darrel. R. (2024): Amphibian Species of the World: an online reference. American Museum of Natural History, New York, USA. Available: https://amphibiansoftheworld.amnh.org/index.php. [Accessed: 02 February 2024]
- Griffiths, R.A. (1986): Feeding niche overlap and food selection in Smooth and Palmate newts, Triturus vulgaris and T. helveticus, at a pond in mid-Wales. J. Anim. Ecol. 55: 201-214.
- Hirai, T., Matsui, M. (2001): Diet composition of the Indian rice frog, Rana limnocharis, in rice fields of central Japan. Curr. Herpetol. 20: 97-103.
- Irschick, D.J., Vanhooydonck, B., Herrel, A., Meyers, J.J. (2005): Intraspecific correlations among morphology, performance, and habitat use within a green anole lizard (Anolis carolinensis) population. Biol. J. Linn. Soc. 85: 211-221.
- Isacch, J.P., Barg, M. (2002): Are bufonid toads specialized ant feeders? A case test from the Argentinian flooding pampa. J. Nat. Hist. 36: 2005-2012.
- IUCN. (2023): The IUCN Red List of Threatened Species. Available: https://www.iucnredlist.org [Accessed: 10 January 2023]
- Johnson, N.F., Triplehorn, C.A. (2005): Borror and Delong’s Introduction to the Study of Insects. Belmont, California, USA, Thomson Learning Inc.
- Krebs, C.J. (1999): Ecological Methodology. Menlo Park, California, Addison Wesley Longman.
- Le, D.M., Raxworthy, C.J., McCord, W.P., Mertz, L. (2006): A molecular phylogeny of tortoises (Testudines: Testudinidae) based on mitochondrial and nuclear genes. Mol. Phylogenet. Evol. 40: 517-531.
- Le, T.T.D., Rowley, J.L.J., Tran, T.A.D., Vo, N.T., Hoang, D.H. (2018): Diet composition and overlap in a montane frog community in Vietnam. Herpetol. Conserv. Biol. 13: 205-215.
- Leclerc, J., Courtois, D. (1993): A simple stomach flushing method for ranid frogs. Herpetol. Rev. 24: 142-143.
- Liu, S., Yang, B., Wang, Q., Hou, M. (2021): Taxonomic reassessment of the poorly known microhylid, Kalophrynus menglienicus Yang & Su, 1980. Herpetozoa. 34: 223-232.
- Lopes, M.S., Bovendorp, R.S., Moraes, G.J.D., Percequillo, A.R., Bertoluci, J. (2017): Diversity of ants and mites in the diet of the Brazilian frog Chiasmocleis leucosticta (Anura: Microhylidae). Biota Neotrop. 17: 1-5.
- Magnusson, W.E., Lima, A.P., Silva, W.A., Araújo, M.C. (2003): Use of geometric forms to estimate volume of invertebrates in ecological studies of dietary overlap. Copeia. 1: 13-19.
- Magurran, A.E. (2004): Measuring Biological Diversity. Malden, Massachusetts, USA, Blackwell Science.
- Maneyro, R.D.E., Naya, I.R., Canavero, A., Camargo, A. (2004): Diet of the South American frog Leptodactylus ocellatus (Anura, Leptodactylidae) in Uruguay. Iheringia Sér. Zool. 94: 57-61.
- Muñoz-Pedreros, A., Merino, C. (2014): Diversity of aquatic bird species in a wetland complex in southern Chile. J. Nat. Hist. 48: 1453-1465.
- Naumann, I.D., Came, P.B., Lawrence, J.F., Nielsen, E.S., Spradbery, J.P., Taylor, R.W., Whitten, M.J., Littlejohn, M.J., Eds. (1991): The Insects of Australia. 2 Vols, Division of Entomology, CSIRO, Australia. Melbourne University Press, Melbourne, Australia, and Cornell University Press, Ithaca, New York.
- Norval, G., Huang, S.C., Mao, J.J., Goldberg, S.R., Yang, Y.J. (2014): Notes on the diets of five amphibian species from southwestern Taiwan. Alytes. 30: 69-77.
- Ohler, A., Grosjean, S. (2005): Color pattern and call variation in Kalophrynus from south-east Asia. Herpetozoa. 18: 99-106.
- Oliveira, M.C.L.M., Santos, M.B., Loebmann, D., Hartman, A., Tozetti, A.M. (2013): Diversity and associations between coastal habitats and anurans in southernmost Brazil. An. Acad. Bras. Cienc. 85: 575-584.
- Palumbi, S.R., Martin, A., Romano, S., McMillan, W.O., Stice, L., Grabowski, G. (1991): The simple fool’s guide to PCR. Department of Zoology and Kewalo Marine Laboratory, Hawaii, USA.
- Pham, V.A., Ngo, D.C., Nguyen, Q.T., Ngo, V.B. (2022): Diet of Microhyla butleri and M. heymonsi from Son La Province, Northwest, Vietnam. Herpetol. Conserv. Biol. 17: 217-224.
- Pham, V.A., Nguyen, Q.T., Nguyen, L.H.S., Tu, V.H., Song, B.N., Giang, A. H. (2016): Diversity of the Narrow-mouth frogs (Amphibia: Anura: Microhylidae) from Son La Province. Proceedings of the 2st National scientific conference on Biological research and teaching in Vietnam. 2: 37-42.
- Prado, C.P.A., Uetanabaro, M., Haddad, C.F.B. (2005): Breeding activity patterns, reproductive modes, and habitat use by anurans (Amphibia) in a seasonal environment in the Pantanal, Brazil. Amphib.-Reptil. 26: 211-221.
- RStudio Team. (2018): RStudio: Integrated Development for R. RStudio, Inc., Boston, MA, USA. Available at: https://www.rstudio.com/.
- Schoener, T.W. (1974): Resource partitioning in ecological communities. Science. 185: 27-39.
- Simmons, J.E. (2002): Herpetological Collecting and Collections Management, 2nd ed. Lawrence, Kansas: Society for the Study of Amphibians and Reptile.
- Solé, M., Beckmann, O., Pelz, B., Kwet, A., Egels, W. (2005): Stomach-flushing for diet analysis in anuran: an improved protocol evaluated in a case study in Araucaria forests, southern Brazil. Stud. Neotrop. Fauna Environ. 40: 23-28.
- Solé, M., Ketterl, J., Di-Bernardo, M., Kwet, A. (2002): Ants and termites are the diet of the microhylid frog Elachistocleis ovalis (Schneider, 1799) at an Araucaria forest in Rio Grande do Sul, Brazil. Herpetol. Bull. 79: 14-17.
- Toft, C.A. (1980): Feeding ecology of thirteen syntopic species of anuran in a seasonal tropical environment. Oecologia. 45: 131-141.
- Toledo, L.F., Silva, R.R., Haddad, C.F.B. (2007): Anurans as prey: an exploratory analysis and size relationships between predators and their prey. J. Zool. 271: 170-177.
- van Schingen, M., Pham, C.T., An, H.T., Nguyen, T.Q., Bernardes, M., Bonkowski, M., Ziegler, T. (2015): First ecological assessment of the endangered crocodile lizard Shinisaurus crocodilurus Ahl, 1930 in Vietnam: Microhabitat characterization and habitat selection. Herpetol. Conserv. Biol. 10: 948-958.
- Wells, K.D. (2007): The Ecology and Behavior of Amphibians. Chicago, Illinois, USA, University of Chicago Press..
- Yang, D.T., Su, C.Y. (1980): A new species of the family Microhylidae frog from Yunnan. Zool. Res. 1: 257-260.