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Dinner Time in the Atlantic Forest: Trophic Niche Partitioning of Two Sympatric Boana Species in the Southern Bahia State, Brazil

Amanda Sabino Martins
Universidade Estadual de Santa Cruz
Kássio de Castro Araújo
Universidade Estadual do Maranhão
Heitor Zochio Fischer
Universidade de Sorocaba
Mirco Solé
Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz - UESC, Campus Soane Nazaré de Andrade, Rod. Jorge Amado, Km 16 - Salobrinho, CEP 45662-900, Ilhéus, Brasil
Danilo Silva Ruas
Universidade Estadual do Sudoeste da Bahia
Caio Vinícius de Mira-Mendes
Universidade Estadual do Maranhão
Iuri Ribeiro Dias
Universidade Estadual de Santa Cruz

Published 2026-02-09

Keywords

  • Amphibian,
  • Diet composition,
  • Feeding habit,
  • Hylidae

How to Cite

Sabino Martins, A., de Castro Araújo, K., Zochio Fischer, H., Solé, M., Silva Ruas, D., de Mira-Mendes, C. V., & Ribeiro Dias, I. (2026). Dinner Time in the Atlantic Forest: Trophic Niche Partitioning of Two Sympatric Boana Species in the Southern Bahia State, Brazil. Acta Herpetologica. https://doi.org/10.36253/a_h-18506

Abstract

Understanding the ecological traits of species, such as dietary habits, is important for elucidating their natural history and ecological interactions. Thus, we investigated the trophic ecology and niche partitioning of two treefrog species (Boana atlantica and B. semilineata) in an Atlantic Forest fragment in southern Bahia State, northeastern Brazil. For that, we obtained the diet of 39 B. atlantica and 25 B. semilineata through stomachal flushing. We found in these two hylids 72 prey items organized into 11 categories, with plant material and Araneae as the most consumed prey. Although the niche breadth of B. atlantica was slightly higher than B. semilineata, these hylids had a substantial trophic niche overlap. Our findings provide novel insights into the feeding ecology of these endemic Atlantic Forest treefrogs, enhancing our understanding of their ecological roles and trophic resource partitioning in this biodiversity hotspot.

References

  1. Anderson, A.M., Haukos, D.A., Anderson, J.T. (1999): Diet composition of three anurans from the Playa Wetlands of Northwest Texas. Copeia. 1999: 515-520. DOI: https://doi.org/10.2307/1447502
  2. Araújo, K.C., Cassiano-Lima, D., Brasileiro, A.C., Bezerra, C.H., Ávila, R.W. (2023): What the minute shield frogs Adelophryne eat at a humid forest relict in Ceará state, northeastern Brazil. North-West. J. Zool. 19: e231503.
  3. Araújo, F.R.R.C., Bocchiglieri, A., Holmes, R.M. (2007): Ecological aspects of the Hypsiboas albopunctatus (Anura, Hylidae) in central Brazil. Neotrop. Biol. Conserv. 2: 165-168.
  4. Blanco‐Torres, A., Duré, M.I., Bonilla, M.A., Cagnolo, L. 2020. Predator–prey interactions in anurans of the tropical dry forests of the Colombian Caribbean: A functional approach. Biotropica. 2020: 1-8. DOI: https://doi.org/10.1111/btp.12779
  5. Caldas, F.L., Garda, A.A., Cavalcanti, L.B., Leite-Filho, E., Faria, R.G., Mesquita, D.O. 2019. Spatial and trophic structure of anuran assemblages in environments with different seasonal regimes in the Brazilian Northeast Region. Copeia. 107: 567-584. DOI: https://doi.org/10.1643/CH-18-109
  6. Camurugi, F., Juncá, F. (2013): Reproductive biology of Hypsiboas atlanticus (Anura: Hylidae). Herpetol. Notes. 6: 489-495.
  7. Castro, I.M., Reboucas, R., Sole, M. (2016): Diet of Dendropsophus branneri (Cochran, 1948) (Anura: Hylidae) from a cocoa plantation in southern Bahia, Brazil. North-West. J. Zool. 12: 159-165.
  8. Ceron, K., Oliveira‐Santos, L.G.R., Souza, C.S., Mesquita, D.O., Caldas, F.L., Araujo, A.C., Santana, D.J. (2019): Global patterns in anuran–prey networks: structure mediated by latitude. Oikos. 128: 1537-1548. DOI: https://doi.org/10.1111/oik.06621
  9. Colón-Gaud, C., Whiles, M.R., Kilham, S.S., Lips, K.R., Pringle, C.M., Connelly, S., Peterson, S.D. (2009): Assessing ecological responses to catastrophic amphibian declines: Patterns of macroinvertebrate production and food web structure in upland Panamanian streams. Limnol. Oceanogr. 54: 331-343. DOI: https://doi.org/10.4319/lo.2009.54.1.0331
  10. Csardi, G., Nepusz, T. (2006): The igraph software package for complex network research. InterJ. Complex Syst. 1695: 1-9.
  11. Das, I. 1996. Folivory and seasonal changes in diet in Rana hexadactyla (Anura: Ranidae). J. Zool. 238: 785-794. DOI: https://doi.org/10.1111/j.1469-7998.1996.tb05430.x
  12. Dormann, C.F., Gruber, B., Fründ, J. (2009): Introducing the bipartite package: Analysing ecological networks. R News. 8: 8-11.
  13. Egeter, B., Bishop, P.J., Robertson, B.C. (2015): Detecting frogs as prey in the diets of introduced mammals: A comparison between morphological and DNA‐based diet analyses. Mol. Ecol. Resour. 15: 306-316. DOI: https://doi.org/10.1111/1755-0998.12309
  14. Ferreira, J.L.P., Araújo, K.C., Andrade, E.B., Mira-Mendes, C.V. (2024): Are the males of Dendropsophus minutus (Peters, 1872) spider devourers?. Cuad. Herpetol. 38:1-5. DOI: https://doi.org/10.31017/CdH.2024.(2024-014)
  15. Flesher, K.M. (2014): Plano de manejo da RPPN Ouro Verde. Igrapiúna: Reserva Ecológica Michelin. Available at: https://purchasing.michelin.com/wp-content/uploads/2025/01/RPPN-Ouro-Verde-Plano-de-Manejo-Sep-17-2014.pdf. /. [Accessed 28 July 2025].
  16. Frost, D.R. (2025): Amphibian species of the world 6.2, an online reference. American Museum of Natural History, New York. Available online at: https://amphibiansoftheworld.amnh.org/. [Accessed 28 July 2025].
  17. Gotelli, N.J. (2009): Ecologia. 4rd Edition. Londrina, Editora Planta.
  18. Heyer, W.R., Donnelly, M.A.R., Mcdiarmid, W., Hayek, L.A.C., Foster, M.S. (1994): Measuring and Monitoring Biological Diversity – Standard Methods for Amphibians. Washington, Smithsonian Institution Press.
  19. Huckembeck, S., Loebmann, D., Albertoni, E. F., Hefler, S. M., Oliveira, M. C., Garcia, A. M. (2014): Feeding ecology and basal food sources that sustain the Paradoxal frog Pseudis minuta: a multiple approach combining stomach content, prey availability, and stable isotopes. Hydrobiologia. 740: 253-264. DOI: https://doi.org/10.1007/s10750-014-2022-2
  20. Hutchinson, G.E. (1957): Concluding Remarks. Cold Spring Harb. Symp. Quant. Biol. 22: 415-427. DOI: https://doi.org/10.1101/SQB.1957.022.01.039
  21. Leite-Filho, E., Oliveira, F.A., Eloi, F.J., Liberal, C.N., Lopes, A.O., Mesquita, D.O. (2017): Evolutionary and ecological factors influencing an anuran community structure in an Atlantic Rainforest urban Fragment. Copeia. 105: 64-74. DOI: https://doi.org/10.1643/CH-15-298
  22. Levins, R. (1968): Evolution in Changing Environments: Some Theoretical Explorations. Princeton, Princeton University Press. DOI: https://doi.org/10.1515/9780691209418
  23. Lima, A.P., Moreira, G. (1993): Effects of prey size and foraging mode on the ontogenetic change in feeding niche of Colostethus stepheni (Anura: Dendrobatidae). Oecologia. 95: 93-102. DOI: https://doi.org/10.1007/BF00649512
  24. López, J.A., Scarabotti, P.A., Medrano, M.C., Ghirardi, R. (2009): Is the red spotted green frog Hypsiboas punctatus (Anura: Hylidae) selecting its preys?: The importance of prey availability. Rev. Biol. Trop. 57: 847-857. DOI: https://doi.org/10.15517/rbt.v57i3.5497
  25. Luría-Manzano, R., Ramírez-Bautista, A. (2019): Ontogenetic variation in the diet of the anuran community from a semi-arid environment in the southeastern Chihuahuan Desert. PeerJ. 7: e7908. DOI: https://doi.org/10.7717/peerj.7908
  26. Macarthur, R.H., Levins, R. (1967): The Limiting Similarity, Convergence, and Divergence of Coexisting Species. Am. Nat. 101: 377-385. DOI: https://doi.org/10.1086/282505
  27. Mahan, R.D., Johnson, J.R. (2007): Diet of the Gray Treefrog (Hyla versicolor) in Relation to Foraging Site Location. J. Herpetol. 41: 16-23. DOI: https://doi.org/10.1670/0022-1511(2007)41[16:DOTGTH]2.0.CO;2
  28. Moroti, M.T., Soares, P.T., Pedrozo, M., Provete, D.B., Santana, D.J. (2021): The effects of morphology, phylogeny and prey availability on trophic resource partitioning in an anuran community. Basic Appl. Ecol. 50: 181-191. DOI: https://doi.org/10.1016/j.baae.2020.11.005
  29. Moser, C.F., Avila, F.R., Oliveira, M., Tozetti, A.M. (2017): Diet composition and trophic niche overlap between two sympatric species of Physalaemus (Anura, Leptodactylidae, Leiuperinae) in a subtemperate forest of southern Brazil. Herpetol. Notes. 10: 9-15.
  30. Moser, C.F., Oliveira, M.D., Avila, F.R.D., Dutra-Araújo, D., Farina, R.K., Tozetti, A.M. (2018): Diet and trophic niche overlap of Boana bischoffi and Boana marginata (Anura: Hylidae) in southern Brazil. Biota Neotrop. 19: e20180542. DOI: https://doi.org/10.1590/1676-0611-bn-2018-0542
  31. Napoli, M.F., Cruz, I.C. (2005): The advertisement call of Hyla atlantica Caramaschi & Velosa, 1996, with considerations on its taxonomic status. Arq. Mus. Nac. 63: 283-288.
  32. Oksanen, J., Blanchet, F.G., Friendly, M., Kindt, R., Legendre, P., McGlinn, D., Minchin, P.R., O’Hara, R.B., Simpson, G.L., Solymos, P., Henry, M., Stevens, H., Szoecs, E., Wagner, H. (2019): Vegan: Community Ecology Package. R package version 2.5-6. Available online at: https://CRAN.R-project.org/package=vegan. [Accessed 28 July 2025].
  33. Oliveira, M., Gottschalk, M.S., Loebmann, D., Santos, M.B., Miranda, S., Rosa, C., Tozetti, A.M. (2015): Diet composition and niche overlap in two sympatric species of Physalaemus (Anura, Leptodactylidae, Leiuperinae) in coastal subtemperate wetlands. Herpetol. Notes. 8: 173-177.
  34. Oliveira-Nogueira, C.H., Souza, U.F., Machado, T.M., Figueiredo-de-Andrade, C.A., Mônico, A.T., Sazima, I., Sazima, M., Toledo, L.F. (2023): Between fruits, flowers and nectar: The extraordinary diet of the frog Xenohyla truncata. Food Webs. 35: e00281. DOI: https://doi.org/10.1016/j.fooweb.2023.e00281
  35. Pacheco, E.O., Ferreira, V.G., Carvalho, R.M.H. (2017): Diet of Boana albopunctata (Anura: Hylidae) in an Atlantic Forest fragment of southeastern Brazil. Phyllomedusa. 16: 57-62. DOI: https://doi.org/10.11606/issn.2316-9079.v16i1p57-62
  36. Parmelee, J.R. (1999): Trophic ecology of a tropical anuran assemblage. Nat. Hist. Mus. Univ. Kans. 11: 1-59. DOI: https://doi.org/10.5962/bhl.title.16167
  37. Pianka, E.R. (1973): The structure of lizard communities. Annu. Rev. Ecol. Syst. 4: 53-74. DOI: https://doi.org/10.1146/annurev.es.04.110173.000413
  38. Pinkas, L., Oliphant, M.S., Iverson, I.L.K. (1971): Food Habits of Albacore, Bluefin tuna, and Bonito in California Waters. Fish. Bull. 152: 1-105.
  39. Poulin, B., Lefebvre, G., Ibáñez, R., Jaramillo, C., Hernández, C., Rand, A.S. (2001): Avian predation upon lizards and frogs in a neotropical forest understorey. J. Trop. Ecol. 17: 21-40. DOI: https://doi.org/10.1017/S026646740100102X
  40. Protázio, A.S., Albuquerque, R.L., Falkenberg, L.M., Mesquita, D.O. (2015): Niche differentiation of an anuran assemblage in temporary ponds in the Brazilian semiarid Caatinga: influence of ecological and historical factors. Herpetol. J. 25: 109-121.
  41. Protázio, A.S., Anunciação, M.V.D.S., Braga, H.S.N., Conceição, L.C., Santos, U.G., Ribeiro, A.C., Cruz, S.J., Protázio, A.S. (2017): Relação de nicho espacial e alimentar entre Boana semilineata e Boana pombali (Anura: Hylidae) em fragmentos de Mata Atlântica no Nordeste da Bahia. Magistra. 29: 315-327.
  42. Rafael, J.A., Melo, G.A.R.D., Carvalho, C.J.B.D., Casari, S.A., Constantino, R. (2024): Insetos do Brasil: diversidade e taxonomia. 2. ed. Manaus: Instituto Nacional de Pesquisas da Amazônia.
  43. Richter-Boix, A., Llorente, G.A., Montori, A., Garcia, J. (2007): Tadpole diet selection varies with the ecological context in predictable ways. Basic Appl. Ecol. 8: 464-474. DOI: https://doi.org/10.1016/j.baae.2006.09.009
  44. Rodrigues, N.L.A., Araújo, K.C., Ávila, R.W., Andrade, E.B. (2023): Feeding habits of Boana raniceps (Cope, 1862) in three mountains of Brazilian semiarid. Univers. Sci. 28: 257-277. DOI: https://doi.org/10.11144/Javeriana.SC282.fhob
  45. Rosa, I., Canavero, A., Maneyro, R., Camargo, A. (2011): Trophic niche variation and individual specialization in Hypsiboas pulchellus (Duméril and Bibron, 1841) (Anura, Hylidae) from Uruguay. South Am. J. Herpetol. 6: 98-106. DOI: https://doi.org/10.2994/057.006.0208
  46. Rossa-Feres, D.C., Garey, M.V., Caramaschi, U., Napoli, M.F., Nomura, F., Bispo, A.A., Hadadd, C. (2017): Anfíbios da Mata Atlântica: Lista de Espécies, Histórico dos Estudos, Biologia e Conservação. Rev. Zool. Mata Atlântica, Sér. Pesq. 310: 231-314.
  47. Sabagh, L.T., Ferreira, V.L., Rocha, C.F.D. (2010): Living together, sometimes feeding in a similar way: the case of the syntopic hylid frogs Hypsiboas raniceps and Scinax acuminatus (Anura: Hylidae) in the Pantanal of Miranda, Mato Grosso do Sul State, Brazil. Braz. J. Biol. 70: 955-959. DOI: https://doi.org/10.1590/S1519-69842010000500006
  48. Sant'anna, B.S., Fonseca, A.M., Ferreira, R.F.B. (2022): Diet composition and coexistence of Boana geographica and Boana raniceps (Anura: Hylidae) from Central Amazonia, Brazil. North-West. J. Zool. 18: 170-183.
  49. Santos, E.M., Almeida, A.V., Vasconcelos, S.D. (2004): Feeding habits of six anuran (Amphibia: Anura) species in a rainforest fragment in Northeastern Brazil. Iheringia. S. Zool. 94: 433-438. DOI: https://doi.org/10.1590/S0073-47212004000400014
  50. Silva, I.C.O., Soares, P., Ribas, A.C.A., Santana, D.J., Campiao, K.M., Tavares, L.E.R. (2021): Scinax fuscovarius (Anura, Hylidae) diet assessment at Serra da Bodoquena, Mato Grosso do Sul, Brazil. North-West. J. Zool. 17: 29-33.
  51. Silva, H.R., Britto-Pereira, M.C., Caramaschi, U. (1989): Frugivory and seed dispersal by Hyla truncata, a Neotropical tree-frog. Copeia. 1989: 785-794. DOI: https://doi.org/10.2307/1445517
  52. Solé, M., Beckmann, O., Pelz, B., Kwet, A., Engels, W. (2005): Stomach-flushing for diet analysis in anurans: an improved protocol evaluated in a case study in Araucaria forests, southern Brazil. Stud. Neotrop. Fauna Environ. 40: 23-28. DOI: https://doi.org/10.1080/01650520400025704
  53. Solé, M., Rödder, D. (2009): Dietary assessments of adult amphibians. In: Amphibian Ecology and Conservation: A Handbook of Techniques, pp. 167-184. Dodd, K.C., Eds, Oxford University Press. DOI: https://doi.org/10.1093/oso/9780199541188.003.0010
  54. Toft, C.A. (1981): Feeding ecology of Panamanian litter anurans: patterns in diet and foraging mode. J. Herpetol. 15: 139-144. DOI: https://doi.org/10.2307/1563372
  55. Tupy, G.S., Cardoso, G.S., Vilanova-Júnior, J.L., Pinto, R.S.S., Vieira, S.S., Gomes, V.M.S., Rodrigues, W.S., Faria, R.G., Caldas, F.L.S. (2021): Trophic ecology of Boana albomarginata and Boana pombali (Anura: Hylidae) during the dry season in the Serra de Itabaiana National Park, Northeast Brazil, North-West. J. Zool. 17: 220-226.
  56. Veloso, H.P., Rangel-Filho, A.L.R., Lima, J.C. (1991): Classificação da vegetação brasileira, adaptada a um sistema universal. Rio de Janeiro, IBGE.
  57. Wells, K.D. (2010): The ecology and behavior of amphibians. University of Chicago Press.
  58. Zhang, J. (2016): Spaa: Species association analysis. R package version 0.2.2. Available online at: https://CRAN.R-project.org/package=spaa. [Accessed 28 July 2025].