Vol. 21 No. 1 (2026)
Articles

Dinner time in the Atlantic Forest: Trophic niche partitioning of two sympatric Boana species in the Southern Bahia State, Brazil

Amanda S. Martins
Programa de Pós-graduação em Ecologia e Conservação da Biodiversidade, Universidade Estadual de Santa Cruz, Ilhéus - UESC, Campus Soane Nazaré de Andrade, Rod. Jorge Amado, Km 16 - Salobrinho, CEP 45662-900, Ilhéus, Brasil
Kássio C. Araújo
Universidade Estadual do Maranhão - UEMA, Cidade Universitária Paulo VI, Av. Lourenço Vieira da Silva, n° 1000, Bairro: Jardim São Cristóvão, CEP 65055-310, São Luís, Brasil
Heitor Z. Fischer
Universidade de Sorocaba - UNISO, Av. Independência, 210 - Éden, CEP 18087-101, Sorocaba, Brasil
Mirco Solé
Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz - UESC, Campus Soane Nazaré de Andrade, Rod. Jorge Amado, Km 16 - Salobrinho, CEP 45662-900, Ilhéus, Brasil
Danilo S. Ruas
Universidade Estadual do Sudoeste da Bahia - UESB, Estrada do Bem Querer, km 4, CEP 45083-900, Vitória da Conquista, Brasil
Caio V. de Mira-Mendes
Universidade Estadual do Maranhão - UEMA, Cidade Universitária Paulo VI, Av. Lourenço Vieira da Silva, n° 1000, Bairro: Jardim São Cristóvão, CEP 65055-310, São Luís, Brasil
Iuri R. Dias
Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz - UESC, Campus Soane Nazaré de Andrade, Rod. Jorge Amado, Km 16 - Salobrinho, CEP 45662-900, Ilhéus, Brasil

Published 2026-02-09

Keywords

  • Amphibian,
  • Diet composition,
  • Feeding habit,
  • Hylidae

How to Cite

Martins, A. S., Araújo, K. C., Fischer, H. Z., Solé, M., Ruas, D. S., de Mira-Mendes, C. V., & Dias, I. R. (2026). Dinner time in the Atlantic Forest: Trophic niche partitioning of two sympatric Boana species in the Southern Bahia State, Brazil. Acta Herpetologica, 21(1), 73–80. https://doi.org/10.36253/a_h-18506

Abstract

Understanding the ecological traits of species, such as dietary habits, is important for elucidating their natural history and ecological interactions. Thus, we investigated the trophic ecology and niche partitioning of two treefrog species (Boana atlantica and B. semilineata) in an Atlantic Forest fragment in southern Bahia, northeastern Brazil. In this study, we obtained the diet of 39 B. atlantica and 25 B. semilineata through stomach flushing. We recorded 72 prey items in these two hylids organized into 11 categories. The most consumed prey were plant material and Araneae. Although the niche breadth of B. atlantica was higher than that of B. semilineata, these hylids had a substantial trophic niche overlap (Pianka’s niche overlap index = 0.89). Our findings provide novel insights into the feeding ecology of these endemic Atlantic Forest treefrogs, enhancing our understanding of their ecological roles and trophic resource partitioning in this biodiversity hotspot.

References

  1. Anderson, A.M., Haukos, D.A., Anderson, J.T. (1999): Diet composition of three anurans from the Playa Wetlands of Northwest Texas. Copeia 1999: 515-520. DOI: https://doi.org/10.2307/1447502
  2. Araújo, K.C., Cassiano-Lima, D., Brasileiro, A.C., Bezerra, C.H., Ávila, R.W. (2023): What the minute shield frogs Adelophryne eat at a humid forest relict in Ceará state, northeastern Brazil. North-West. J. Zool. 19: e231503.
  3. Araújo, F.R.R.C., Bocchiglieri, A., Holmes, R.M. (2007): Ecological aspects of the Hypsiboas albopunctatus (Anura, Hylidae) in central Brazil. Neotrop. Biol. Conserv. 2: 165-168.
  4. Blanco‐Torres, A., Duré, M.I., Bonilla, M.A., Cagnolo, L. (2020): Predator–prey interactions in anurans of the tropical dry forests of the Colombian Caribbean: A functional approach. Biotropica 2020: 1-8. DOI: https://doi.org/10.1111/btp.12779
  5. Burnham, K.P., Anderson, D.R. (2002): Model selection and multimodel inference: A practical information-theoretic approach. New York, Springer-Verlag.
  6. Caldas, F.L., Garda, A.A., Cavalcanti, L.B., Leite-Filho, E., Faria, R.G., Mesquita, D.O. (2019): Spatial and trophic structure of anuran assemblages in environments with different seasonal regimes in the Brazilian Northeast Region. Copeia. 107: 567-584. DOI: https://doi.org/10.1643/CH-18-109
  7. Camurugi, F., Juncá, F. (2013): Reproductive biology of Hypsiboas atlanticus (Anura: Hylidae). Herpetol. Notes 6: 489-495.
  8. Caramaschi, U., Velosa, A. (1996): Nova espécie de Hyla Laurenti, 1768 do leste brasileiro (Amphibia, Anura, Hylidae). Bol. Mus. Nac., N. Ser., Zool. 365:1-7.
  9. Castro, I.M., Rebouças, R., Solé, M. (2016): Diet of Dendropsophus branneri (Cochran, 1948) (Anura: Hylidae) from a cocoa plantation in southern Bahia, Brazil. North-West. J. Zool. 12: 159-165.
  10. Ceron, K., Oliveira‐Santos, L.G.R., Souza, C.S., Mesquita, D.O., Caldas, F.L., Araujo, A.C., Santana, D.J. (2019): Global patterns in anuran–prey networks: structure mediated by latitude. Oikos 128: 1537-1548. DOI: https://doi.org/10.1111/oik.06621
  11. Colón-Gaud, C., Whiles, M.R., Kilham, S.S., Lips, K.R., Pringle, C.M., Connelly, S., Peterson, S.D. (2009): Assessing ecological responses to catastrophic amphibian declines: Patterns of macroinvertebrate production and food web structure in upland Panamanian streams. Limnol. Oceanogr. 54: 331-343. DOI: https://doi.org/10.4319/lo.2009.54.1.0331
  12. Csardi, G., Nepusz, T. (2006): The igraph software package for complex network research. InterJ. Complex Syst. 1695: 1-9.
  13. Das, I. (1996): Folivory and seasonal changes in diet in Rana hexadactyla (Anura: Ranidae). J. Zool. 238: 785-794. DOI: https://doi.org/10.1111/j.1469-7998.1996.tb05430.x
  14. Dormann, C.F., Gruber, B., Fründ, J. (2009): Introducing the bipartite package: Analysing ecological networks. R News 8: 8-11.
  15. Egeter, B., Bishop, P.J., Robertson, B.C. (2015): Detecting frogs as prey in the diets of introduced mammals: A comparison between morphological and DNA‐based diet analyses. Mol. Ecol. Resour. 15: 306-316. DOI: https://doi.org/10.1111/1755-0998.12309
  16. Ferreira, J.L.P., Araújo, K.C., Andrade, E.B., Mira-Mendes, C.V. (2024): Are the males of Dendropsophus minutus (Peters, 1872) spider devourers? Cuad. Herpetol. 38:1-5. DOI: https://doi.org/10.31017/CdH.2024.(2024-014)
  17. Flesher, K.M. (2014): Plano de manejo da RPPN Ouro Verde. Igrapiúna: Reserva Ecológica Michelin. Available at: https://purchasing.michelin.com/wp-content/uploads/2025/01/RPPN-Ouro-Verde-Plano-de-Manejo-Sep-17-2014.pdf. [Accessed 28 July 2025].
  18. Frost, D.R. (2025): Amphibian species of the world 6.2, an online reference. American Museum of Natural History, New York.
  19. Gause, G.F. (1932): Experimental studies on the struggle for existence I. Mixed population of two species of yeast. J. Exp. Biol. 9:389-402. DOI: https://doi.org/10.1242/jeb.9.4.389
  20. Gotelli, N.J. (2009): Ecologia. 4th Edition. Londrina, Editora Planta.
  21. Heyer, W.R., Donnelly, M.A., McDiarmid, W., Hayek, L.A.C., Foster, M.S. (1994): Measuring and monitoring biological diversity – Standard methods for amphibians. Washington, Smithsonian Institution Press.
  22. Huckembeck, S., Loebmann, D., Albertoni, E. F., Hefler, S. M., Oliveira, M. C., Garcia, A. M. (2014): Feeding ecology and basal food sources that sustain the Paradoxal frog Pseudis minuta: A multiple approach combining stomach content, prey availability, and stable isotopes. Hydrobiologia 740: 253-264. DOI: https://doi.org/10.1007/s10750-014-2022-2
  23. Hutchinson, G.E. (1957): Concluding remarks. Cold Spring Harb. Symp. Quant. Biol. 22: 415-427. DOI: https://doi.org/10.1101/SQB.1957.022.01.039
  24. Izecksohn, E., Carvalho, S.P. (2001): Anfíbios do município do Rio de Janeiro. Rio de Janeiro, Editora UFRJ.
  25. James, G., Witten, D., Hastie, T., Tibshirani, R. (2013): An introduction to statistical learning: With applications in R. New York, Springer. DOI: https://doi.org/10.1007/978-1-4614-7138-7
  26. Kneitel, J.M. (2019): Gause’s competitive exclusion principle. In: Encyclopedia of ecology, pp. 110-113. Fath, B.D, Eds, Elsevier. DOI: https://doi.org/10.1016/B978-0-12-409548-9.00816-2
  27. Leite-Filho, E., Oliveira, F.A., Eloi, F.J., Liberal, C.N., Lopes, A.O., Mesquita, D.O. (2017): Evolutionary and ecological factors influencing an anuran community structure in an Atlantic Rainforest urban Fragment. Copeia 105: 64-74. DOI: https://doi.org/10.1643/CH-15-298
  28. Levins, R. (1968): Evolution in changing environments: some theoretical explorations. Princeton, Princeton University Press. DOI: https://doi.org/10.1515/9780691209418
  29. Lima, A.P., Moreira, G. (1993): Effects of prey size and foraging mode on the ontogenetic change in feeding niche of Colostethus stepheni (Anura: Dendrobatidae). Oecologia 95: 93-102. DOI: https://doi.org/10.1007/BF00649512
  30. López, J.A., Scarabotti, P.A., Medrano, M.C., Ghirardi, R. (2009): Is the red spotted green frog Hypsiboas punctatus (Anura: Hylidae) selecting its preys?: The importance of prey availability. Rev. Biol. Trop. 57: 847-857. DOI: https://doi.org/10.15517/rbt.v57i3.5497
  31. Luría-Manzano, R., Ramírez-Bautista, A. (2019): Ontogenetic variation in the diet of the anuran community from a semi-arid environment in the southeastern Chihuahuan Desert. PeerJ 7: e7908. DOI: https://doi.org/10.7717/peerj.7908
  32. MacArthur, R.H., Levins, R. (1967): The limiting similarity, convergence, and divergence of coexisting species. Am. Nat. 101: 377-385. DOI: https://doi.org/10.1086/282505
  33. Machado, H.T.S., Silva, C.F., Benício, R.A., Ávila, R.W. (2024): Feeding ecology, reproductive biology, and sexual dimorphism of Boana raniceps (Anura: Hylidae) in an area of Caatinga, northeastern Brazil. Caldasia 46: 71-80. DOI: https://doi.org/10.15446/caldasia.v46n1.99220
  34. Mahan, R.D., Johnson, J.R. (2007): Diet of the Gray Treefrog (Hyla versicolor) in relation to foraging site location. J. Herpetol. 41: 16-23. DOI: https://doi.org/10.1670/0022-1511(2007)41[16:DOTGTH]2.0.CO;2
  35. Moroti, M.T., Soares, P.T., Pedrozo, M., Provete, D.B., Santana, D.J. (2021): The effects of morphology, phylogeny and prey availability on trophic resource partitioning in an anuran community. Basic Appl. Ecol. 50: 181-191. DOI: https://doi.org/10.1016/j.baae.2020.11.005
  36. Moser, C.F., Avila, F.R., Oliveira, M., Tozetti, A.M. (2017): Diet composition and trophic niche overlap between two sympatric species of Physalaemus (Anura, Leptodactylidae, Leiuperinae) in a subtemperate forest of southern Brazil. Herpetol. Notes 10: 9-15.
  37. Moser, C.F., Oliveira, M.D., Avila, F.R.D., Dutra-Araújo, D., Farina, R.K., Tozetti, A.M. (2018): Diet and trophic niche overlap of Boana bischoffi and Boana marginata (Anura: Hylidae) in southern Brazil. Biota Neotrop. 19: e20180542. DOI: https://doi.org/10.1590/1676-0611-bn-2018-0542
  38. Moser, C.F., Ceron, C., Schuck, L.K., Santana, D.J., Solé, M., Iop, S., Tozetti, A.M. (2025): Current state of the art in anuran dietary studies in Brazil. Discov. Ecol. 1: 1-9. DOI: https://doi.org/10.1007/s44396-025-00008-7
  39. Müller, K., Wickham, H. (2023): Tibble: Simple data frames. R package version 3.3.0.
  40. Naimi, B., Hamm, N.A.S., Groen, T.A., Skidmore, A.K., Toxopeus, A.G. (2014): Where is positional uncertainty a problem for species distribution modelling?. Ecography 37:191-203. DOI: https://doi.org/10.1111/j.1600-0587.2013.00205.x
  41. Napoli, M.F., Cruz, I.C. (2005): The advertisement call of Hyla atlantica Caramaschi & Velosa, 1996, with considerations on its taxonomic status. Arq. Mus. Nac. 63: 283-288.
  42. Oksanen, J., Blanchet, F.G., Friendly, M., Kindt, R., Legendre, P., McGlinn, D., Minchin, P.R., O’Hara, R.B., Simpson, G.L., Solymos, P., Henry, M., Stevens, H., Szoecs, E., Wagner, H. (2019): Vegan: Community ecology package. R package version 2.5-6.
  43. Oliveira, M., Gottschalk, M.S., Loebmann, D., Santos, M.B., Miranda, S., Rosa, C., Tozetti, A.M. (2015): Diet composition and niche overlap in two sympatric species of Physalaemus (Anura, Leptodactylidae, Leiuperinae) in coastal subtemperate wetlands. Herpetol. Notes 8: 173-177.
  44. Oliveira-Nogueira, C.H., Souza, U.F., Machado, T.M., Figueiredo-de-Andrade, C.A., Mônico, A.T., Sazima, I., Sazima, M., Toledo, L.F. (2023): Between fruits, flowers and nectar: The extraordinary diet of the frog Xenohyla truncata. Food Webs 35: e00281. DOI: https://doi.org/10.1016/j.fooweb.2023.e00281
  45. Pacheco, E.O., Ferreira, V.G., Carvalho, R.M.H. (2017): Diet of Boana albopunctata (Anura: Hylidae) in an Atlantic Forest fragment of southeastern Brazil. Phyllomedusa 16: 57-62. DOI: https://doi.org/10.11606/issn.2316-9079.v16i1p57-62
  46. Parmelee, J.R. (1999): Trophic ecology of a tropical anuran assemblage. Nat. Hist. Mus. Univ. Kans. 11: 1-59. DOI: https://doi.org/10.5962/bhl.title.16167
  47. Pedersen, T.L. (2022): ggraph: An implementation of grammar of graphics for graphs and networks. R package version 2.2.2.
  48. Pianka, E.R. (1973): The structure of lizard communities. Annu. Rev. Ecol. Syst. 4: 53-74. DOI: https://doi.org/10.1146/annurev.es.04.110173.000413
  49. Pinkas, L., Oliphant, M.S., Iverson, I.L.K. (1971): Food habits of albacore, bluefin tuna, and bonito in California waters. Fish. Bull. 152: 1-105.
  50. Poulin, B., Lefebvre, G., Ibáñez, R., Jaramillo, C., Hernández, C., Rand, A.S. (2001): Avian predation upon lizards and frogs in a neotropical forest understorey. J. Trop. Ecol. 17: 21-40. DOI: https://doi.org/10.1017/S026646740100102X
  51. Protázio, A.S., Albuquerque, R.L., Falkenberg, L.M., Mesquita, D.O. (2015): Niche differentiation of an anuran assemblage in temporary ponds in the Brazilian semiarid Caatinga: Influence of ecological and historical factors. Herpetol. J. 25: 109-121.
  52. Protázio, A.S., Vieira, M.V.D.S.A., Braga, H.S.N., Conceição, L.C., Santos, U.G., Ribeiro, A.C., Cruz, S.J., Protázio, A.S. (2017): Relação de nicho espacial e alimentar entre Boana semilineata e Boana pombali (Anura: Hylidae) em fragmentos de Mata Atlântica no Nordeste da Bahia. Magistra. 29: 315-327.
  53. Rafael, J.A., Melo, G.A.R.D., Carvalho, C.J.B.D., Casari, S.A., Constantino, R. (2024): Insetos do Brasil: Diversidade e taxonomia. 2nd ed. Manaus: Instituto Nacional de Pesquisas da Amazônia.
  54. Richter-Boix, A., Llorente, G.A., Montori, A., Garcia, J. (2007): Tadpole diet selection varies with the ecological context in predictable ways. Basic Appl. Ecol. 8: 464-474. DOI: https://doi.org/10.1016/j.baae.2006.09.009
  55. Rodrigues, N.L.A., Araújo, K.C., Ávila, R.W., Andrade, E.B. (2023): Feeding habits of Boana raniceps (Cope, 1862) in three mountains of Brazilian semiarid. Univers. Sci. 28: 257-277. DOI: https://doi.org/10.11144/Javeriana.SC282.fhob
  56. Rosa, I., Canavero, A., Maneyro, R., Camargo, A. (2011): Trophic niche variation and individual specialization in Hypsiboas pulchellus (Duméril and Bibron, 1841) (Anura, Hylidae) from Uruguay. South Am. J. Herpetol. 6: 98-106. DOI: https://doi.org/10.2994/057.006.0208
  57. Rossa-Feres, D.C., Garey, M.V., Caramaschi, U., Napoli, M.F., Nomura, F., Bispo, A.A., Haddad, C. (2017): Anfíbios da Mata Atlântica: Lista de espécies, histórico dos estudos, biologia e conservação. Rev. Zool. Mata Atlântica, Sér. Pesq. 310: 231-314.
  58. Sabagh, L.T., Ferreira, V.L., Rocha, C.F.D. (2010): Living together, sometimes feeding in a similar way: The case of the syntopic hylid frogs Hypsiboas raniceps and Scinax acuminatus (Anura: Hylidae) in the Pantanal of Miranda, Mato Grosso do Sul State, Brazil. Braz. J. Biol. 70: 955-959. DOI: https://doi.org/10.1590/S1519-69842010000500006
  59. Sant’anna, B.S., Fonseca, A.M., Ferreira, R.F.B. (2022): Diet composition and coexistence of Boana geographica and Boana raniceps (Anura: Hylidae) from Central Amazonia, Brazil. North-West. J. Zool. 18: 170-183.
  60. Santos, E.M., Almeida, A.V., Vasconcelos, S.D. (2004): Feeding habits of six anuran (Amphibia: Anura) species in a rainforest fragment in Northeastern Brazil. Iheringia. S. Zool. 94: 433-438. DOI: https://doi.org/10.1590/S0073-47212004000400014
  61. Silva, I.C.O., Soares, P., Ribas, A.C.A., Santana, D.J., Campiao, K.M., Tavares, L.E.R. (2021): Scinax fuscovarius (Anura, Hylidae) diet assessment at Serra da Bodoquena, Mato Grosso do Sul, Brazil. North-West. J. Zool. 17: 29-33.
  62. Silva, H.R., Britto-Pereira, M.C., Caramaschi, U. (1989): Frugivory and seed dispersal by Hyla truncata, a Neotropical tree-frog. Copeia 1989: 785-794. DOI: https://doi.org/10.2307/1445517
  63. Solé, M., Beckmann, O., Pelz, B., Kwet, A., Engels, W. (2005): Stomach-flushing for diet analysis in anurans: An improved protocol evaluated in a case study in Araucaria forests, southern Brazil. Stud. Neotrop. Fauna Environ. 40: 23-28. DOI: https://doi.org/10.1080/01650520400025704
  64. Solé, M., Rödder, D. (2009): Dietary assessments of adult amphibians. In: Amphibian Ecology and Conservation: A Handbook of Techniques, pp. 167-184. Dodd, K.C., Ed, Oxford University Press. DOI: https://doi.org/10.1093/oso/9780199541188.003.0010
  65. Toft, C.A. (1981): Feeding ecology of Panamanian litter anurans: Patterns in diet and foraging mode. J. Herpetol. 15: 139-144. DOI: https://doi.org/10.2307/1563372
  66. Tupy, G.S., Cardoso, G.S., Vilanova-Júnior, J.L., Pinto, R.S.S., Vieira, S.S., Gomes, V.M.S., Rodrigues, W.S., Faria, R.G., Caldas, F.L.S. (2021): Trophic ecology of Boana albomarginata and Boana pombali (Anura: Hylidae) during the dry season in the Serra de Itabaiana National Park, Northeast Brazil, North-West. J. Zool. 17: 220-226.
  67. Veloso, H.P., Rangel-Filho, A.L.R., Lima, J.C. (1991): Classificação da vegetação brasileira, adaptada a um sistema universal. Rio de Janeiro, IBGE.
  68. Wells, K.D. (2010): The ecology and behavior of amphibians. University of Chicago Press.
  69. Wickham H (2016) ggplot2: Elegant graphics for data analysis. 3rd ed. New York, Springer-Verlag, 2016. DOI: https://doi.org/10.1007/978-3-319-24277-4
  70. Zhang, J. (2016): Spaa: Species association analysis. R package version 0.2.2.