Vol. 35 No. 3 (2021): Advances in Horticultural Science
Articles

Punica granatum L. extract contributes to phytopathogens control and enhances Eruca vesicaria (L.) Cav. germination in vitro and in vivo

S. De Sousa Silva
Departamento de Ciências Naturais, Universidade Federal do Maranhão, Estrada Pinheiro/Pacas, Km 10, s/n, Enseada, 65200-000 Pinheiro, Maranhão, Brazil
P.C.S. Alves
Centro de Ciências Biológicas e da Saúde, Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, Cidade Universitária do Bacanga, Avenida dos Portugueses, 1966, 65080-805 São Luís, Maranhão, Brazil
D.F. Coutinho
Departamento de Farmácia, Laboratório de Farmacognosia II, Universidade Federal do Maranhão, Cidade Universitária do Bacanga, Avenida dos Portugueses, 1966, 65080-805 São Luís, Maranhão, Brazil
T.R.S.A. Luz
Laboratório de Imunofisiologia, Universidade Federal do Maranhão, Cidade Universitária do Bacanga, Avenida dos Portugueses, 1966, 65080-805 São Luís, Maranhão, Brazil
G.M.G. Fontoura
Laboratory of Research, Development and Innovation, Apis Flora Indl. Coml. Ltda., Rua Triunfo, 945, Santa Cruz, 14020-670 Ribeirão Preto, São Paulo, Brazil
A.A. Berretta
Laboratory of Research, Development and Innovation, Apis Flora Indl. Coml. Ltda., Rua Triunfo, 945, Santa Cruz, 14020-670 Ribeirão Preto, São Paulo, Brazil.
M.C. Gonçalves Maciel
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Bloco E s/n 1° andar - Asa Norte, 70910-900, Brasília, Distrito Federal, Brazil
Published May 26, 2021
Keywords
  • antimicrobial,
  • botanical pesticides,
  • food security,
  • plant diseases,
  • pomegranate peel,
  • punicalagin
  • ...More
    Less
How to Cite
Silva, S. de S., Alves, P. C. dos S., Coutinho, D. F., Luz, T. R. S. A., Fontoura, G. M. G., Berretta, A. A., & Gonçalves Maciel, M. C. (2021). Punica granatum L. extract contributes to phytopathogens control and enhances Eruca vesicaria (L.) Cav. germination in vitro and in vivo. Advances in Horticultural Science, 35(3), 217-231. https://doi.org/10.36253/ahsc-10706

Abstract

The study aimed to investigate antimicrobial activity of the hydroalcoholic crude extract from the fruit peel of Punica granatum (Pp) and punicalagin compound (Pg) on phytopathogenic bacterial isolates and its potential use as a sustainable alternative in treatment of vegetable seeds. The antimicrobial activity in vitro was tested by agar well diffusion assay and through viability tests in liquid medium. In vivo treatment with Pp was tested on Eruca vesicaria seeds infected with Xanthomonas campestris pv. campestris. Pp induced the formation of large inhibition zones to the growth of the tested pathogens (35.33 mm – 6.66 mm), with dose-dependent effect. Viability tests confirmed the antimicrobial activity of the Pp on X. campestris pv. campestris and P. carotovorum subsp. carotovorum with minimum inhibitory concentration (MIC) of 125 μg/mL. Punicalagin compound presented MIC of the 31.25 μg/mL. The seed treatment with Pp indicated control of pathogen-induced symptoms in seedlings of the E. vesicaria and positive effect in seed germination, emergence and in stomatal functionality. The results indicate strong potential of the extract from the fruit peel of P. granatum and Punicalagin for formulating botanical pesticides for plant disease control.

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