Vol. 35 No. 3 (2021): Advances in Horticultural Science
Articles

Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.

S. Kivrak Kiran
Molecular Biology and Genetics Department, Faculty of Science, Mugla Sitki Kocman University, 48000 Mugla, Turkey
S. Galatali
Molecular Biology and Genetics Department, Faculty of Science, Mugla Sitki Kocman University, 48000 Mugla, Turkey
S. Yeniocak
Molecular Biology and Genetics Department, Faculty of Science, Mugla Sitki Kocman University, 48000 Mugla, Turkey
D.E. Ozkaya
Molecular Biology and Genetics Department, Faculty of Science, Mugla Sitki Kocman University, 48000 Mugla, Turkey
T. Mercan
Molecular Biology and Genetics Department, Faculty of Science, Mugla Sitki Kocman University, 48000 Mugla, Turkey
S. Guldag
Molecular Biology and Genetics Department, Faculty of Science, Mugla Sitki Kocman University, 48000 Mugla, Turkey
O. Celik
Molecular Biology and Genetics Department, Faculty of Science, Mugla Sitki Kocman University, 48000 Mugla, Turkey
N. Abdul Ghafoor
Molecular Biology and Genetics Department, Faculty of Science, Mugla Sitki Kocman University, 48000 Mugla, Turkey
E. Kaya
Molecular Biology and Genetics Department, Faculty of Science, Mugla Sitki Kocman University, 48000 Mugla, Turkey
Published August 3, 2021
Keywords
  • Charcoal,
  • Fe-EDDHA,
  • hazelnut,
  • ISSR,
  • micropropagation
How to Cite
Kivrak Kiran, S., Galatali, S., Yeniocak, S., Ozkaya, D. E., Mercan, T., Guldag, S., Celik, O., Abdul Ghafoor, N., & Kaya, E. (2021). Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L. Advances in Horticultural Science, 35(3), 285-292. https://doi.org/10.36253/ahsc-10536

Abstract

Cultivation of Corylus avellana L. in Turkey is performed generally in the northern regions where it is an important source of livelihood for the local farmers. More than 70% of world hazelnut production is supplied by Turkey, but compared with other countries, Turkey’s hazelnut production area is quite narrow. In this study was aimed to develop an effective in vitro production for seven local cultivars of C. avellana. Therefore, WPM medium supplemented with 6-Benzylaminopurine (BAP) was modified by using single or in combination of Fe-EDDHA, AgNO3, H3BO3, charcoal and gibberellic acid. In all varieties, the best regeneration rates varying between 68% and 94% were obtained from WPM medium supplemented with 4.4 µM BAP, 27.8 µM Fe-EDDHA and 10g/L Charcoal. Genetic stability of shoots derived from meristem culture using the best medium was analysed using ISSR primers, when the gel images of the PCR products were examined, no polymorphic band was observed in samples collected from seven provinces, and the genetic stability was determined as 100%.

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