Vol. 28 No. 2 (2014): Special issue on Current Environmental and Horticultual Research Progress in Japan
Articles

Molecular cloning and characterization of ABCG/PDR-type ABC transporter in grape berry skin

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  • M. Suzuki,
  • M. Jasinski,
  • E. Martinoia,
  • R. Nakabayashi,
  • M Suzuki,
  • K. Saito,
  • K. Shiratake
M. Suzuki
Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601
M. Jasinski
Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd, 60-637 Poznan'
E. Martinoia
University of Zurich, Institute of Plant Biology, Zollikerstrasse, CH-8008 Zurich
R. Nakabayashi
RIKEN Center for Sustainable Resource Science, Suehiro-cho, Tsurumi-ku, Yokohama 230-0045
M Suzuki
RIKEN Center for Sustainable Resource Science, Suehiro-cho, Tsurumi-ku, Yokohama 230-0045
K. Saito
Graduate School of Pharmaceutical Sciences, Chiba University, Inohana, Chuo-ku, Chiba 260-8675
K. Shiratake
Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601
DOI: https://doi.org/10.13128/ahs-22795

Published 2014-06-30

Keywords

  • full-size ABCG transporter,
  • gene expression,
  • grape,
  • resveratrol

How to Cite

Suzuki, M., Jasinski, M., Martinoia, E., Nakabayashi, R., Suzuki, M., Saito, K., & Shiratake, K. (2014). Molecular cloning and characterization of ABCG/PDR-type ABC transporter in grape berry skin. Advances in Horticultural Science, 28(2), 53–63. https://doi.org/10.13128/ahs-22795
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Abstract

Grape (Vitis vinifera L.) skin contains the phenolic compound resveratrol which is important not only for resistance to biotic and abiotic stresses but also for human health. However, little is known about resveratrol transport in plant cells. ABC (ATP binding cassette) transporters are well-known transporters responsible for secondary metabolite accumulation in plants. Previous reports speculated that the full-size ABCG transporter pleiotropic drug-resistant (PDR) is involved in resveratrol transport in fungi and plants. In this paper, all full-size ABCG transporters found in the grape genome database are listed and focus is placed on VvABCG44/VvPDR14 as a candidate resveratrol transporter. The full-length cDNA of VvABCG44 was cloned by RT-PCR using mRNAs extracted from grape berry skin. VvABCG44 expression was induced by UV irradiation, and the expression pattern of VvABCG44 in various grape organs was similar to that of stilbene synthase (STS), a key enzyme in resveratrol synthesis. Resveratrol content in grape berry skin increased after UV irradiation. These results suggest that VvABCG44 functions as a resveratrol transporter in grape.

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