Vol. 28 No. 2 (2014): Special issue on Current Environmental and Horticultual Research Progress in Japan
Articles

Development of in vitro propagation by node culture and cryopreservation by V-Cryo-plate method for Perilla frutescens

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  • T. Matsumoto,
  • K. Yoshimatsu,
  • N. Kawahara,
  • S.-I. Yamamoto,
  • T. Niino
T. Matsumoto
Faculty of Life and Environmental Sciences, Shimane University, Matsue 690-1102
K. Yoshimatsu
Tsukuba Division, Research Center for Medicinal Plant Resources, National Institution of Biomedical Innovation, Tsukuba 305-0843
N. Kawahara
Tsukuba Division, Research Center for Medicinal Plant Resources, National Institution of Biomedical Innovation, Tsukuba 305-0843
S.-I. Yamamoto
National Institute of Agrobiological Sciences (NIAS), Tsukuba 305-8602
T. Niino
Gene Research Center, University of Tsukuba, Tsukuba 305-8572
DOI: https://doi.org/10.13128/ahs-22798

Published 2014-06-30

Keywords

  • aluminum plate,
  • cryopreservation,
  • Perilla

How to Cite

Matsumoto, T., Yoshimatsu, K., Kawahara, N., Yamamoto, S.-I., & Niino, T. (2014). Development of in vitro propagation by node culture and cryopreservation by V-Cryo-plate method for Perilla frutescens. Advances in Horticultural Science, 28(2), 79–83. https://doi.org/10.13128/ahs-22798
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Abstract

A clonal propagation method by node culture of perilla (Perilla frutescens L. Britton) was investigated. Nodes were plated on solidified 1/2 MS medium with BA and optimum shoot elongation and propagation was obtained at BA 0.05 and 0.1 mg l-1. Cryopreservation using an aluminum cryo-plate was successfully applied to in vitro-grown perilla shoot tips. Excised shoot tips from nodes were precultured on 1/2 MS medium with 0.3 M sucrose and embedded on an aluminum cryo-plate with alginate gel. The cryo-plate with shoot tips was osmo-protected with LS solution and dehydrated in PVS2 for 20 min at 25°C prior to immersion into liquid nitrogen. The recovery growth after cryopreservation was found to be about 80%. This new V-Cryo-plate method has many advantages and may facilitate the cryo-storage of other medicinal plants.

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