Vol. 30 No. 1 (2016)
Articles

DNA changes in cotton (<em>Gossypium hirsutum</em> L.) under salt stress as revealed by RAPD marker

Published 2016-07-27

Keywords

  • cotton,
  • genomic template stability,
  • RAPD

How to Cite

Saleh, B. (2016). DNA changes in cotton (<em>Gossypium hirsutum</em> L.) under salt stress as revealed by RAPD marker. Advances in Horticultural Science, 30(1), 13–21. https://doi.org/10.13128/ahs-18697

Abstract

Random amplified polymorphic DNA (RAPD) analysis was applied to evaluate DNA changes among four upland cotton (Gossypium hirsutum L.) varieties [Niab 78 (N78), Deir-Ezzor 22 (DE22), Deltapine 50 (DP50) and Aleppo 118 (A118)] grown under non-saline conditions (control) and salt stress (200 mM NaCl) for seven weeks. Changes in RAPD profiles were measured as genomic template stability (GTS%). The highest estimated GTS% value was recorded for the two sensitive varieties, DP50 (79.1%) followed by A118 (58.2%); whereas, the lowest value was recorded for the two other tolerant varieties DE22 (36.7%) followed by N78 (26.4%). Based upon the data presented, RAPD marker could be used as potential tool for early identification of cotton tolerance to salt stress.

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