Improvement of in vitro germination of Cycas revoluta zygotic embryos using gelrite as gelling agent
Copyright (c) 2020 Jalila Benjelloun, aida Taoufyq, Zine El Abidine Triqui, Qamar Lahlimi, Rajaa Layachi, Abdelaziz Smouni, Sarah Bouzroud, Abdelkarim Guedira
This work is licensed under a Creative Commons Attribution 4.0 International License.
An efficient in vitro germination protocol for Cycas revoluta, a widespread ornamental tree, has been established using zygotic embryos as explants with a focus on mineral composition of the culture media, the gelling agent and cytokinine type. A high percentage of germination, 73% was obtained with SH medium instead of 27% with MS medium. A 100% of germination was obtained with the combination of SH medium and gelrite as gelling agent. The addition of cytokinines prompt shoot formation. An optimum shoot induction occurred using 0.5 mg/l of BAP where an average of 14.1 shoot were produced per explants while 2.2 shoots were formed in the presence of 2iP. A 100% of rooting was observed in the presence of 0.5 mg/l of 2iP whereas only 30% of shoots obtained on the SH medium with 0.5 mg/l of BAP were able to develop roots.