Vol. 38 No. 4 (2024):
Articles

In vitro propagation and shootlets assessment for drought and salinity tolerance of traditional accessions of potato

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  • A. Elbitar,
  • A. Chehade,
  • F. Kanj,
  • S. Yahfoufi
A. Elbitar
Lebanese Agricultural Research Institute, Tal Amara Station, Department of Plant Biotechnology, Plant Tissue Culture Unit, PO.Box 287, Zahleh, Lebanon.
A. Chehade
Lebanese Agricultural Research Institute, Tal Amara Station, Department of Plant Biotechnology, Plant Tissue Culture Unit, PO Box 287, Zahleh, Lebanon.
F. Kanj
Lebanese University, Faculty of Agronomy and Veterinary Sciences, Department of Environment, Dekwaneh, Lebanon.
S. Yahfoufi
Lebanese University, Faculty of Agronomy and Veterinary Sciences, Department of Environment, Dekwaneh, Lebanon.
DOI: https://doi.org/10.36253/ahsc-16275
Categories

Published 2025-03-10

Keywords

  • Mannitol,
  • micropropagation,
  • sodium chloride,
  • Solanum tuberosum L.,
  • temperature

How to Cite

Elbitar, A., Chehade, A., Kanj, F., & Yahfoufi, S. (2025). In vitro propagation and shootlets assessment for drought and salinity tolerance of traditional accessions of potato . Advances in Horticultural Science, 38(4), 351–362. https://doi.org/10.36253/ahsc-16275

Copyright (c) 2024 Suzanne YAHFOUFI

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

Abiotic stresses, including heat, drought, and salinity, pose severe threats to agricultural yields, globally affecting essential crops like potatoes. The aim of this study is to establish an in vitro culture system for three potato accessions: Tal Amara 1 (TA1), Tal Amara 2 (TA2), and Tal Amara 3 (TA3) and to quantify their tolerance to temperature, drought, salinity, and combined stresses. The results demonstrated that MS0 (devoid of growth regulators) medium was the best for culture initiation, with a percentage of reactive meristems of 82.22%, whereas MS1 (0.35 mg L-1 Kin + 0.2 mg L-1 IAA + 0.1 mg L-1 GA3) medium resulted in the highest multiplication rate of 5.5. The most heat tolerant accession was TA1, with shootlets lengths ranging from 2 cm to 4.4 cm at temperatures of 4°C and 38°C respectively. Concerning the effect of combined drought and temperature stresses, TA1 and TA3 showed tolerance to the different mannitol concentrations. Likewise, the most prominent accession in terms of combined salinity and temperature tolerance was TA2, with shootlets lengths of 3.2 cm (60 Mm NaCl, 22°C), 2.03 cm (60 Mm NaCl, 4°C) and 1.6 cm (60 Mm NaCl, 38°C).

 

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