
Published 2025-08-08
Keywords
- Anthocyanins,
- carotenoids,
- chlorophyll,
- cultivars,
- flavonoids
- polyphenols ...More
How to Cite
Copyright (c) 2025 Priyanganie Senanayake, Dilki Thathsarani, Thilakshi Tikirikumari, Asha Priyadarshan, Harshini Herath

This work is licensed under a Creative Commons Attribution 4.0 International License.
Funding data
-
Ministry of Agriculture
Grant numbers NARP/21/UK/SC/01
Abstract
The genus Phalaenopsis in family Orchidaceae has gained popularity in the global floriculture market for its value as potted plants and cut flowers. Hybridization plays a pivotal role in breeding Phalaenopsis, enabling the development of novel cultivars with desirable traits such as diverse floral pigmentation patterns, enhanced longevity and improved growth rate. The selection of suitable parental cultivars is a crucial determinant in the hybridization process, as it plays a key role in defining pigmentation patterns and enhancing desirable traits for the development of superior cultivars. This research investigated the phytochemical composition, including carotenoids, anthocyanins, flavonoids and phenolics contents of selected commercial Phalaenopsis cultivars. Using UV-vis spectrophotometry, the chlorophylls, flavonoids, carotenoids, anthocyanins and phenolics contents in the flowers, leaves and roots of six cultivars were analyzed quantitatively. The results revealed significant variations in phytochemical properties across the tested cultivars and plant organs. Phalaenopsis cvs. Taipei Gold Gold Star, Red Lip 1770, Golden Sands Canary, Sogo Yukidian V3 and Queen Beer Mantefon exhibited promising phytochemical profiles. Notably, cvs. Taipei Gold Gold Star, Golden Sands Canary and Queen Beer Mantefon were identified as ideal parental candidates for hybridization due to their potential to develop distinctive floral colorations and robust vegetative traits. These findings provide valuable insights into the floral coloration and the phytochemical richness of vegetative parts in Phalaenopsis cultivars. This knowledge can contribute to the development of innovative, high-quality cultivars with enhanced survival rates and greater consumer appeal.
References
- AXIOTIS E., ANGELIS A., ANTONIADI L., PETRAKIS E.A., SKALTSOUNIS L.A., 2021 - Phytochemical analysis and dermo-cosmetic evaluation of Cymbidium sp. (Orchidaceae) cultivation by-products. - Antioxidants, 11(1): 101.
- BHARDWAJ R., KUMAR M., KAUSHAL N., KAMBOJ A.D., KRISHNAMOORTHI A., SINGH A., MOTLA R., 2024 - From Lab to Bouquet: The biotechnological frontier in modern floriculture for sustainable and resilient flower farming. - J. Adv. Biol. Biotechnol., 27(2): 119-137.
- CARDOSO J.C., VENDRAME W.A., 2022 - Innovation in propagation and cultivation of ornamental plants. -Horticulturae, 8(3): 229.
- CARDOSO J.C., VILCHERREZ-ATOCHE J.A., IIYAMA C.M., GERMANÀ M.A., VENDRAME W.A., 2023 - Breeding of Orchids using conventional and biotechnological methods: advances and future prospects, pp. 27-58. - In: TIWARI P., and J.T. CHENG (eds.) Advances in orchid biology, Biotechnology and omics. Springer Nature, Singapore, pp. 283.
- CARLSEN S.C., MORTENSEN AG., OLESZEK W., PIACENTE S., STOCHMAL A., FOMSGAARD I.S., 2008 - Variation in flavonoids in leaves, stems and flowers of white clover cultivars. - Nat. Prod. Commun., 3(8): 1299-1306.
- CAVAIUOLO M., FERRANTE A., CIRIELLO G., 2013 - The role of polyphenols in delaying senescence and enhancing the functional and aesthetic lifespan of plant organs. - J. Plant Biol., 68(4): 276-283.
- CHASE M., CAMERON K., FREUDENSTEIN J., PRIDGEON A., SALAZAR G., VAN DEN BERG C., SCHUITEMAN A., 2015 - An updated classification of Orchidaceae - Bot. J. Linn. Soc., 177(2): 151-174.
- DARRAS A.I., 2020 - Implementation of sustainable practices to ornamental plant cultivation worldwide: A critical review. - Agronomy, 10(10): 1570.
- EBRAHIMI A., ASADI A., MONFARED S.R., SAHEBI M., REZAEE S., KHALEDIAN Y., 2020 - Evaluation of phenotypic diversity of the endangered orchid (Orchis mascula): Emphasizing on breeding, conservation and development. - S. Afr. J. Bot., 132: 304-315.
- HE T., FAN X., HUANG T., ZHANG Y., 2023 - Phytochemical diversity of cultivated Dendrobium nobile lindl. in Southwestern China and its association with environmental conditions. - Appl. Ecol. Environ. Res., 21(5): 3923-3942.
- HSIAO Y., PAN Z., HSU C., YANG Y., HSU Y., CHUANG Y., SHIH H., CHEN W., TSAI W., CHEN H., 2011 - Research on orchid biology and biotechnology. - Plant Cell Physiol., 52(9): 1467-1486.
- HSU C., CHEN H., CHEN W., 2018 - Phalaenopsis, pp. 567-625. - In J. HUYLENBROECK (ed.) Ornamental crops. Springer International Publishing, Cham, Switzerland, pp. 887.
- LALROSANGPUII, LALROKIMI, 2021 - A review on the phytochemical properties of five selected genera of orchids. - Sci Vis., 21(2): 50-58.
- LATTANZIO V., CARDINALI A., LINSALATA V., 2012 - Plant Phenolics: A biochemical and physiological perspective, pp. 1-39. – In: CHEYNIER V., P. SARNI-M, and S. QUIDEAU (eds.) Recend advances in polyphenol research. John Wiley & Sons, Ltd, Ames, IA, USA, pp. 364.
- LEE Y., TSENG Y., LEE Y., CHUNG M., 2020 - Chromosome constitution and nuclear DNA content of Phalaenopsis hybrids - Sci. Hortic., 262:109089.
- LIGHTBOURN G., GRIESBACH R., NOVOTNY J., CLEVIDENCE B., RAO D., STOMMEL J., 2008 - Effects of anthocyanin and carotenoid combinations on foliage and immature fruit color of Capsicum annuum L - J. Hered., 99(2): 105-111.
- LIN M., HSU B., 2004 - Photosynthetic plasticity of Phalaenopsis in response to different light environments - J. Plant Physiol., 161(11): 1259-1268.
- LING L., SUBRAMANIAM S., 2007 - Biochemical analyses of Phalaenopsis violacea orchids. - Asian J. Biochem., 2(4): 237-246.
- MARTIN C., MAS E., LU C., ONG B., 2010 - The photosynthetic pathway of the roots of twelve epiphytic orchids with CAM leaves. - Photosynthetica., 48(1): 42-50.
- MINH T.N., KHANG D.T., TUYEN P.T., MINH L.T., ANH L.H., QUAN N.V., HA P.T.T., QUAN N.T., TOAN N.P., ELZAAWELY A.A., XUAN T.D., 2016 - Phenolic compounds and antioxidant activity of Phalaenopsis orchid hybrids - Antioxidants, 5(3): 31.
- NARBONA E., DEL VALLE J.C., WHITTALL J.B., 2021 - Painting the green canvas: how pigments produce flower colours. - Biochemist, 43(3): 6-12.
- NGUYEN H., KUAN-HUNG L., HUANG M., CHI-MING Y., TIN-HAN S., HSIUNG T., YEN-CHANG L., FUN-CHI T., 2018 - Antioxidant activities of the methanol extracts of various parts of Phalaenopsis orchids with white, yellow, and purple flowers. - Notul. Bot. Hort. Agrobot. Cluj-Napoca, 46(2): 457-465.
- NURCAHYANI E., SHOLEKHAH., SUMARDI., QUDUS H., 2021 - Analysis of total carbohydrate and chlorophyll content of the orchid plantlet [Phalaenopsis amabilis (L.) Bl.] resistant Fusarium wilt disease. - J. Phys. Conf. Ser., 1751: 1-6.
- PORRA R., 2002 - The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b. - Photosynth. Res., 73: 149-156.
- THACKER H., RAM V., 2020 - Role of phytochemicals in plants: A Review. - Int. J. Res. Appl. Sci. Eng. Techn., 8(XII): 19-21.
- TIWARI P., SHARMA A., BOSE S.K., PARK K.I., 2024 - Advances in orchid biology: Biotechnological achievements, translational success, and commercial outcomes. - Horticulturae, 10 (152).
- TRELKA T., BREŚ W., JÓŹWIAK A., KOZŁOWSKA A., 2010 - Phalaenopsis cultivation in different media. Part II. Nutrients and chlorophyll concentration in leaves and roots. - Acta Sci. Pol. Hortorum Cultus, 9(3): 95-104.
- VOEGELPOEL L., 1990 - Flower color. An appreciation. - Orchid Digest., 55: 82-87.
- WANI T.A., BHAT I.A., GULERIA K., FAYAZ M., ANJU T., HARITHA K., KUMAR A., KALOO Z.A., 2022 - Phytochemicals: Diversity, sources and their roles, pp. 3-33. - In: SWAMY M.K., and A. KUMAR (eds.) Phytochemical genomics: plant metabolomics and medicinal plant genomics. Springer Nature, Singapore, pp. 698.