Vol. 40 No. 1 (2026): AHS 150 years
Articles

Estimates of genetic variability and correlation coefficient for yield and its traits in black cumin (Nigella sativa L.) Genotypes in South Wollo Ethiopia

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  • A.E. Seid,
  • Y. Gedamu
A.E. Seid
Department of Horticulture, College of Agriculture and Natural Resource, Mekdela Amba University, Tuluawlia, Ethiopia.
Y. Gedamu
Department of Plant Science and College of Agriculture, Wollo University, Ethiopia. Presente address: Bioinformatics and Genomics Directorate, Bio and Emerging Technology Institute, Addis Ababa, Ethiopia.
DOI: https://doi.org/10.36253/ahsc-16973
Categories

Published 2026-04-07

Keywords

  • Black cumin,
  • genetic advance,
  • genetic variability,
  • genotypic correlation,
  • heritability,
  • Nigella sativa,
  • phenotypic correlation
    • ...More
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How to Cite

Endris Seid, A., & Gedamu, Y. (2026). Estimates of genetic variability and correlation coefficient for yield and its traits in black cumin (Nigella sativa L.) Genotypes in South Wollo Ethiopia. Advances in Horticultural Science, 40(1), 3–18. https://doi.org/10.36253/ahsc-16973

Copyright (c) 2026 Adem Endris

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

Black cumin (Nigella sativa L.) is a valuable annual spice used in food, cosmetics, and industrial products. However, productivity is limited by the lack of improved varieties, mainly due to insufficient knowledge of the genetic diversity within Ethiopian black cumin populations. A study was conducted to assess genetic variability and interrelationships among traits related to seed yield in various black cumin genotypes. Twenty-five genotypes were tested using a 5x5 simple lattice design in Hara, Tehuledere district, Ethiopia during the 2022/2023 growing season. Analysis of variance revealed significant differences for most parameters, except stand count at harvest and thousand seed weight. Broad-sense heritability (H²) and genetic advance as a percentage of the mean (GAM) ranged from 8.24% for days to 90% maturity to 89.56% for the number of tertiary branches per plant, and 4.51% for days to 90% maturity to 84.85% for the number of tertiary branches per plant, respectively. High to moderate estimates of genetic and phenotypic coefficients of variation (GCV and PCV), heritability, and GAM were observed for the number of tertiary branches per plant, number of flowers, number of capsules, biological yield, seed yield, and harvest index. The results also showed significant positive correlations between seed yield and various traits, including plant height, number of primary and tertiary branches, flowers, capsules, and seeds per capsule, biological yield, and harvest index, at both the genotypic and phenotypic levels. The study concluded that significant genetic variability exists, offering potential for targeted selection to enhance specific traits.

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References

  1. ABERA S., HIRKO B., 2020 - Chemical composition of essential oils of released black cumin varieties grown in Ethiopia. - Chem. Mat. Res., 12(2): 9-14.
  2. ADAM A., GETINET A., 2006 - Evaluation of Ethiopian black cumin (Nigella sativa L.) landraces for agronomic characters and oil content at Adet and Woreta, North West Ethiopia. - PhD Thesis, University of Haramaya, pp. 80.
  3. ALLARD R.W., 1960 - Selection under self-fertilization, pp. 1-55. - In: ALLARD R.W. Principles of plant breeding. John Wiley and Sons, Inc., New York, USA, pp. 485.
  4. BADARY O.A., HAMZA M.S., TIKAMDAS R., 2021 - Thymoquinone: A promising natural compound with potential benefits for COVID-19 prevention and cure. - Drug Des. Dev. Ther., 15: 1819-1833.
  5. BAIRWA R.K., SOLANKI R.K., SHARMA Y.K., MEENA R.S., 2015 - Phenotypic variability in cumin (Cuminum cyminum L.) for important agro-morphological traits. - Int. J. Seed Spices., 1: 68-70.
  6. BARDIDEH K., KAHRIZI D., GHOBADI M.H., 2013 - Character association and path analysis of black cumin (Nigella sativa L.) genotypes under different irrigation regimes. - Not. Sci. Biol., 5: 104-108.
  7. BEGNA T., TERESSA T., 2024 - Genetic variability and its benefits in crop improvement: A review. - Middle East J. Agric. Res., 13(1): 128-136.
  8. BITEW J.M., 2016 - Estimation of genetic parameters, heritability and genetic advance for yield related traits in upland rice (Oryza sativa L. and Oryza glaberrima Steud) genotypes in Northwestern Ethiopia. - World Sci. News., 47(2): 340-350.
  9. BURTON G.W., VENE E.M., 1953 - Estimating heritability in tall fescue from replicated clonal material. - Agronomy J., 45: 478-481.
  10. DABROWSKI G., CZAPLICKI S., KONOPKA I., 2024 - Variation in the composition and quality of Nigella sativa L. seed oils: The underestimated impact on possible health-promoting properties. - Molecules, 29(1360): 1-15.
  11. DAWA A., KLEMOLA T., SALONIEMI I., NIEMELÄ P., VUORISALO T., 2018 - Energy for sustainable development factors affecting genetic and seed yield variability of Jatropha curcas ( L .) across the globe: A review. - Energy Sustain. Dev., 42: 170-182.
  12. YIMAM E., NEBIYU A., MOHAMMED A., GETACHEW M., 2015 - Effect of nitrogen and phosphorus fertilizers on growth, yield, and yield components of black bumin (Nigella sativa L.) at Konta District, South West Ethiopia. - J. Agron., 14(3): 112-120.
  13. FARAVANI M., KHAN M.H., GHOLAMI B.A., QAZANCHIAN G.A., SAGHI D.A., 2015 - Variability studies of some black caraway (Bunium persicum bioss. Fedts) accessions across important growing sites of iran. Agric. For., 61(3): 101-110.
  14. FARAVANI M., RAZAVI S.A., FARSI M., 2006 - Study of variation in some agronomic and anatomic characters of Nigella sativa L. landraces in Khorasan. - Iran. J. Med. Aromat. Plants, 22(3): 193-197.
  15. FIKRE D., 2023 - Evaluation of black cumin (Nigella sativa L.) genotypes for yield and yield related parameters in potential growing areas of Ethiopia. - Int. J. Bio-resour. Stress Manag., 14(7): 1037-1045.
  16. FUFA M., 2016 - Correlation studies in yield and some yield components of black cumin (Nigella sativa L.) landraces evaluated at southeastern Ethiopia. - Adv. Crop Sci. Technol., 04(05): 4-5.
  17. GEBREGERGS G., MEKBIB F., 2020 - Estimation of genetic variability, heritability, and genetic advance in advanced lines for grain yield and yield components of sorghum (Sorghum bicolor L. Moench) at Humera, Western Tigray, Ethiopia. - Cogent Food Agric., 6(1): 1-9.
  18. GEBREMEDIN B.D., ASFAW B.T., MENGESHA W.A., ABEBE K.A., 2024 - Genetic diversity of Ethiopian black cumin (Nigella sativa L.) based on morpho-agronomic characteristics. - Euphytica, 220(4): 51.
  19. GELAYE Y., 2025 - Intercropping of pepper (Capsicum annuum L.) and black cumin (Nigella sativa L.) optimize crop performance and system productivity in Ethiopia: Systematic review. - Cogent Food Agric., 11(1): 1-11.
  20. GOMEZ K.A., GOMEZ A.A., 1984 - Statistical procedures for agricultural research. 2nd Edition. - John Wiley and Sons Inc., New York, USA, pp. 680.
  21. HADRU D., ALAMEREW S., ENDALE D., 2014 - Assessment of genetic variability, genetic advance, correlation and path analysis for morphological traits in sesame genotypes. - Asian J. Agric. Res., 8(4): 181-194.
  22. HAMMO Y.H., 2008 - Effect of high levels of nitrogen and phosphorus fertilizer, pinching, and seed rate on growth and yield components of Nigella sativa L. - Mesopotamia J. Agric., 36(1): 19-29.
  23. HAQ M.Z., HOSSAIN M.M., HAQUE M.M., DAS M.R., SHAMSUL HUDA M., 2015 - Blossoming characteristics in black cumin genotypes in relation seed yield influenced by sowing time. - Am. J. Plant Sci., 06(08): 1167-1183.
  24. HERMS S., 2015 - Investment opportunities in the Ethiopian Spices sub-sector. - Advances Consulting, The Netherlands. Report Spices no. 6. Series Ethiopian Netherlands business event 5-6 November, Rijswijk, The Netherlands, pp. 30.
  25. HIKA G., GELETA N., JALETA Z., 2015 - Genetic variability, heritability and genetic advance for the phenotypic traits in sesame (Sesamum indicum L.) populations from Ethiopia. - Sci. Technol. Arts Res. J., 7522: 20-26.
  26. IQBAL M.S., ABDUL G.,MUHAMMAD A., SHAMIM A., SAMMER F., EJAZ H.S., 2019 - Genetic variation and path analysis for yield and other agronomic traits in Nigella sativa L. germplasm. - Bangladesh J. Bot., 48 (3): 521-527.
  27. JANSEN P.C.M., 1981- Spices, condiments and medicinal plants in Ethiopia, their taxonomy and agricultural significance. - Cent. Agric. Publ. Docum. Wageningen, pp. 1-338.
  28. JOHNSON H.W., ROBINSON H.F., COMSTOCK R.E., 1955 - Estimates of genetic and environmental variability in Soybeans. - Agron. J., 47: 314-318.
  29. KASSA M., DAGNE W., DAGNACHEW L., FIREW M., 2019 - Genetic variability of common bean (Phaseolus vulgaris L.) genotypes under sole and maize-bean cropping systems in Bako, Western Oromia, Ethiopia. - Afr. J. Agric. Res., 14(7): 419-429.
  30. KHAN M.L.A., 2009 - Kalonji (Nigella sativa L.). - Islamic Voice, 13-08(152): 1-2.
  31. KUMAR T.N.V., ALLOLI T.B., HADIMANI H., AJJAPPALAVAR P.S., SATISH D., KAREEM A.K., HANCHINAMANI C.N., 2019 - Studies on genetic variability, heritability and genetic advance in garden pea (Pisum sativum L.) varieties. - Int. J. Curr. Microbiol. Appl. Sci., 8(12): 3032-3038.
  32. MANR, 2016 - Plant variety release, protection and seed quality control Directorate. - MANR, Ministry of Agriculture and Natural Reources, Crop variety registered, Addis Abeba, 19: 1-330.
  33. MEENA Y.K., JADHAO B.J., KALE V.S., 2014 - Genetic analysis of agronomic traits in coriander. - SABRAO J. Breed. Genet., 46: 265-273.
  34. MENGESHA B., GETINET G., 2011 - Variability in Ethiopian coriander accessions for agronomic and quality traits. - Afr. Crop Sci. J., 18(2): 43-49.
  35. NADAF N.H., GAWADE S.S., MUNIV A.S., WAGHMARE S.R., JADHAV D.B., SONAWANE K.D., 2015 - Exploring anti-yeast activity of Nigella sativa L. seed extracts. - Ind. Crops Prod., 77: 624-630.
  36. NERGIZ C., ÖTLES S., 1993 - Chemical composition of Nigella sativa L. seeds. - Food Chem., 48: 259-261.
  37. PREETI V., SOLANKI R.K., DASHORA A., KAKANI R.K., 2019 - Genetic variability and correlation analysis in nigella (Nigella sativum L.) assessed in South Eastern Rajasthan, India. - Int. J. Curr. Microbiol. Appl. Sci., 8(03): 1858-1864.
  38. RAO V.S., 2011 - Genetic diversity and conservation of landraces in crop improvement. - Euphytica, 179(2): 303-315.
  39. ROBERTSON A., 1959 - Sampling variance of the genetic correlation coefficient. - Biometrics, 15: 469-485.
  40. SABAGHNIA N., 2025. - Correlation and path analysis of morphologic characters associated with yield performance in black cumin. - J. Plant Biol. Sci., 2(3): 1-13.
  41. SABLE S.V., DESHMUKH D.T., GHAWADE S.M., RAWAT S.S., 2020 - Genetic variability and correlation studies in Garlic (Allium sativum L.). - Int. J. Curr. Microbiol. Appl. Sci., 9(5): 358-363.
  42. SEID H., KEBEDE W., ALEMAW A.G., 2013 - Genetic variability assessment of Ethiopian Caraway. - Adv. Life Sci. Technol., 10: 6-12.
  43. SHARMA J.R., 1998 - Statistical and biometrical techniques in plant breeding. - New Age International (P) Limited Publishers, New Delhi, pp. 432.
  44. SHIMELIS T., 2021 - Spices production and marketing in Ethiopia: A review Spices production and marketing in Ethiopia . - Cogent Food Agric., 7(1): 1-17.
  45. SINGH B.D., 2001 - Plant breeding: Principles and methods. - Kalyani Publishers, New Delhi, India, pp. 896.
  46. SIVASUBRAMANIAH S., MENON M., 1973 - Heterosis and inbreeding depression in rice. - Madras Agric. J., 60: 1139-1144.
  47. TADESSE W., 2015 - Effects of environmental factors on seed yield in agricultural crops. - Environ. Exp. Bot., 117: 116-123.
  48. TESHOME W., ANSHISO D., 2019 - Assessment of production and utilization of black cumin at the Oromia Regional State, Ethiopia. - Asian J. Agric. Ext. Econ. Social., 31: 1-12.
  49. TEWODROS L., MEKBIB F., KEBEBEW A., 2018 - Correlation and path analysis for yield and yield component in black cumin. - Int. J. Curr. Res. Acad. Rev., 6(11): 56-63.
  50. TILAHUN G.W., JALETO K., ALI A., FUFA N., TSAGAYE D., FIKRE D., GEBRETENSAY F., 2024 - Evaluation of Ethiopian white cumin (Trachyspermum ammi L.) accessions for agronomic and quality traits in the Central Highlands of Ethiopia. - Cogent Food Agric., 10(1): 1-9.
  51. TIRU T., WONDIMU B., AREGA G., HASSEN B., 2017 - Spice production, marketing, and utilization in South Wollo, Ethiopia.- East Afr. J. Sci., 11(1): 27-36.
  52. WEISS E., 2004 - Spice crops. - J. Sci. Food Agric., CABI Publishing, Wallingford, UK, pp. 432.
  53. YEWUBDINBER L., 2020 - Genetic variability and association of traits in black cumin (Nigella sativa L.) at Debre-zeit, central Ethiopia. - M.S. Thesis, Haramaya University, Ethiopia, pp. 1-100.
  54. YIMER E.M., TUEM K.B., KARIM A., UR-REHMAN N., ANWAR F., 2019 - Black cumin (Nigella sativa L.). A promising natural remedy for wide range of illnesses. - Evid.-Based Commun. Altern. Med., 1528635: 1-16.
  55. YIMER M., 2010 - Market profile on spices: Ethiopia. - UNCTAD ITC. United Nation Conference on Trade and Development, Addis Ababa, Ethiopia, pp. 1-54.
  56. ZIGYALEW G., GEBRESELASSIE W., HAILEMICHAEL G., 2020 - Correlation and path coefficient analysis in yield and yield related components of black cumin (Nigella Sativa L.) Accessions, at Jimma, Southwest Ethiopia. - Int. J. Agron., 1528635: 1-19.