Vol. 40 No. 3 (2026):
Articles

Auxin-induced plantlet formation of Dendrobium stratiotes x Dendrobium lineale hybrid in in vitro culture

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  • E.W. Tini,
  • Z. Ulinnuha,
  • R.R. Kurniawan,
  • P. Widodo,
  • R. Prasetyo,
  • Sugiyono
E.W. Tini
Faculty of Agriculture Jenderal Soedirman University, Jl. Dr. Soeparno 61 Purwokerto, 53122 Indonesia.
Z. Ulinnuha
Faculty of Agriculture Jenderal Soedirman University, Jl. Dr. Soeparno 61 Purwokerto, 53122 Indonesia.
R.R. Kurniawan
Faculty of Agriculture Jenderal Soedirman University, Jl. Dr. Soeparno 61 Purwokerto, 53122 Indonesia.
P. Widodo
Faculty of Biology Jenderal Soedirman University, Jl. dr. Soeparno 63 Purwokerto, 53122 Indonesia.
R. Prasetyo
Faculty of Science and Technology, Riyadlul Ulum University, Tasikmalaya, West Java, Indonesia.
Sugiyono
Faculty of Biology Jenderal Soedirman University, Jl. dr. Soeparno 63 Purwokerto, 53122 Indonesia.
DOI: https://doi.org/10.36253/ahsc-17170
Categories

Published 2026-10-26

Keywords

  • Crossing,
  • native species,
  • orchid,
  • plant growth regulator

How to Cite

Wukir Tini, E., Ulinnuha, Z., Kurniawan, R., Widodo, P., Prasetyo, R., & Sugiyono, S. (2026). Auxin-induced plantlet formation of Dendrobium stratiotes x Dendrobium lineale hybrid in in vitro culture. Advances in Horticultural Science, 40(3), 247–252. https://doi.org/10.36253/ahsc-17170

Copyright (c) 2026 Etik Wukir Tini

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

Native species orchids, as a source of genetic diversity, can be used in crossing activities to produce hybrid orchids. Dendrobium is a type of orchid in great demand and of high economic value because of its unique flower shape and colour. Developing new varieties through interspecific crossings has produced a D. stratiotes × D. lineale hybrid that successfully germinated in in vitro culture. This experiment was carried out to study the effect of auxin type and concentration on D. stratiotes × D. lineale hybrid orchid plantlet development in in vitro culture, as well as to determine the best type and concentration of auxin to stimulate D. stratiotes × D. lineale hybrid orchid plantlet development. The results showed that the number of shoots formed and the root length of the D. stratiotes × D. lineale hybrid were controlled by the interaction between auxin type and its concentration. Adding 15 µM IBA produced the most shoots, 16.33 shoots per explant. In addition, NAA application resulted in the longest shoots (1.38 cm shoot per explants) and most roots (11 roots per explants) at 8 weeks after planting, which were significantly different from those produced by IAA or IBA.

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