Vol. 28 No. 3 (2014):
Articles

Cultural and genetic evaluation of Cochliobolus sativus during successive passages through suscepible barley

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  • M.I.E. Arabi,
  • M. Jawhar
M.I.E. Arabi
Department of Molecular Biology and Biotechnology, AECS, Damascus, Syria.
M. Jawhar
Department of Molecular Biology and Biotechnology, AECS, Damascus, Syria.
DOI: https://doi.org/10.36253/ahsc-18395
Categories

Published 2014-06-30

Keywords

  • Cochliobolus sativus,
  • Hordeum vulgare,
  • phenotypes,
  • spot blotch

How to Cite

Arabi, M., & Jawhar, M. (2014). Cultural and genetic evaluation of Cochliobolus sativus during successive passages through suscepible barley. Advances in Horticultural Science, 28(3), 119–122. https://doi.org/10.36253/ahsc-18395

Copyright (c) 2014 M.I.E. Arabi, M. Jawhar

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

The objective of this work was to assess the stability of retrotransposons DNA elements and several key phenotypic traits important for virulence of Cochliobolus sativus after serially transferring through susceptible barley plants. A significant increase in virulence was observed in offspring isolates generated from the aggressive isolate Pt4, in contrast to the lack of significant changes in those obtained from the weakly aggressive isolate Pt1 after seven successive passages. No apparent differences in phenotypes, including mycelial growth, conidiation and conidial germination were observed among isolates from the same parent isolate on artificial medium. Based on retrotransposon microsatellite amplified polymorphism (REMAP), parents and their generations were identical during the serial transfers. Taken together, our results suggest that all  singleconidials of the parents and their generations were stable genotypically during seven serial transfers with a change in  virulence of the aggressive isolate generations.

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