Vol. 27 No. 3 (2013): Special issue on "Grapevine nursery"
Short note

Harmonization and validation of diagnostic protocols for the detection of grapevine viruses covered by phytosanitary rules

F. Faggioli
Consiglio per la Ricerca e sperimentazione in Agricoltura, Centro di Ricerca per la Patologia Vegetale, Via C.G. Bertero, 22, 00156 Rome, Italy.
F. Anaclerio
Vivai Cooperativi Rauscedo, Rauscedo, Italy.
E. Angelini
CRA, Centro di Ricerca per la Viticoltura, Conegliano Veneto, Italy.
M.G. Antonelli
Consiglio per la Ricerca e sperimentazione in Agricoltura, Centro di Ricerca per la Patologia Vegetale, Via C.G. Bertero, 22, 00156 Rome, Italy.
N. Bertazzon
CRA, Centro di Ricerca per la Viticoltura, Conegliano Veneto, Italy.
G. Bianchi
ERSA, Friuli Venezia Giulia, Pozzuolo del Friuli, Italy.
P. Bianchedi
Fondazione Edmund Mach, Istituto Agrario San Michele all’Adige, Italy.
P.A. Bianco
DISA, Dipartimento di Scienze agrarie e ambientali, Produzione, Territorio, Agroenergia, Università degli Studi di Milano, Italy.
S. Botti
Centro Attività Vivaistiche, Tebano, Italy.
P. Bragagna
Fondazione Edmund Mach, Istituto Agrario San Michele all’Adige, Italy.
M. Cardoni
Centro Attività Vivaistiche, Tebano, Italy.
P. Casati
DISA, Dipartimento di Scienze agrarie e ambientali, Produzione, Territorio, Agroenergia, Università degli Studi di Milano, Italy.
R. Credi
Dipartimento di Scienze e Tecnologie Agroambientali, Patologia Vegetale, Alma Mater Studiorum, Università di Bologna, Italy.
E. De Luca
Vivai Cooperativi Rauscedo, Rauscedo, Italy.
G. Durante
IPAD Lab, Lodi, Italy.
G. Gianinazzi
IPAD Lab, Lodi, Italy.
G. Gambino
CNR, Istituto di Virologia Vegetale, UOS di Grugliasco, Italy.
V. Gualandri
Fondazione Edmund Mach, Istituto Agrario San Michele all’Adige, Italy.
D. Luison
Consiglio per la Ricerca e sperimentazione in Agricoltura, Centro di Ricerca per la Patologia Vegetale, Via C.G. Bertero, 22, 00156 Rome, Italy.
A. Luvisi
Department of Agriculture, Food and Environment, Università di Pisa, Italy.
U. Malossini
Fondazione Edmund Mach, Istituto Agrario San Michele all’Adige, Italy.
F. Mannini
CNR, Istituto di Virologia Vegetale, UOS di Grugliasco, Italy.
P. Saldarelli
CNR, Istituto di Virologia Vegetale, UOS di Bari, Italy.
F. Terlizzi
Dipartimento di Scienze e Tecnologie Agroambientali, Patologia Vegetale, Alma Mater Studiorum, Università di Bologna, Italy.
E. Triolo
Department of Agriculture, Food and Environment, Università di Pisa, Italy.
N. Trisciuzzi
CRSA, Basile Caramia, Locorotondo, Italy.
M. Barba
Consiglio per la Ricerca e sperimentazione in Agricoltura, Centro di Ricerca per la Patologia Vegetale, Via C.G. Bertero, 22, 00156 Rome, Italy.

Published 2013-09-30

Keywords

  • ELISA test,
  • grapevine nursery,
  • RT-PCR

How to Cite

Faggioli, F., Anaclerio, F., Angelini, E., Antonelli, M., Bertazzon, N., Bianchi, G., Bianchedi, P., Bianco, P., Botti, S., Bragagna, P., Cardoni, M., Casati, P., Credi, R., De Luca, E., Durante, G., Gianinazzi, G., Gambino, G., Gualandri, V., Luison, D., Luvisi, A., Malossini, U., Mannini, F., Saldarelli, P., Terlizzi, F., Triolo, E., Trisciuzzi, N., & Barba, M. (2013). Harmonization and validation of diagnostic protocols for the detection of grapevine viruses covered by phytosanitary rules. Advances in Horticultural Science, 27(3), 107–108. https://doi.org/10.36253/ahsc-18433

Abstract

The Italian Ministry of Agriculture funded in 2009 the “ARNADIA” Project, aimed at producing validated reference diagnostic protocols for the control and monitoring of plant pathogens of phytosanitary interest and, among them, grapevine viruses. In this framework, the “Working group ARNADIA – grapevine viruses (WG)”, composed of eight universities and research bodies, three accredited private laboratories, one plant health service and one association of grapevine nurseries, was established. The aim of the WG was to produce referenced and validated serological and molecular protocols allowing for the harmonization of diagnosis of eight grapevine viruses: Grapevine leafroll-associated virus 1, 2, 3, (GLRaV 1, 2, 3) Grapevine virus A (GVA), Grapevine virus B (GVB), Arabis mosaic virus (ArMV), Grapevine fanleaf virus (GFLV) and Grapevine fleck virus (GFkV). Specifically, 122 grapevine samples (varieties, rootstocks and “pools” of five plants, of which only one infected) were analyzed by ELISA, using 25 antisera from three commercial companies (Agritest, Bioreba, Sediag) and multiplex RTPCR protocols. ELISA proved to be a highly effective technique, comparable to the molecular method, although the latter turned out, as expected, to be more efficient for some viruses and on some specific samples (rootstocks and “pool”).

References

  1. GAMBINO G., GRIBAUDO I., 2006 - Simultaneous detection of nine grapevine viruses by Multiplex Reverse Transcription-Polymerase Chain Reaction with Co-amplification of a plant RNA as internal control. - Phytopathology, 96(11): 1223-1229.