Vol. 28 No. 4 (2014):
Articles

Cross-protection against anthracnose with heat stress, antioxidative changes and proteomic analysis in mycorrhizal cyclamen

pdf
  • S. Watanabe,
  • Y.-I. Matsubara
S. Watanabe
Graduate School of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
Y.-I. Matsubara
Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
DOI: https://doi.org/10.36253/ahsc-18458
Categories

Published 2014-12-31

Keywords

  • Colletotricum gloeosporioides,
  • DPPH,
  • growth promotion,
  • shock heat stress,
  • SOD,
  • symbiosis
    • ...More
    Less

How to Cite

Watanabe, S., & Matsubara, Y.-I. (2014). Cross-protection against anthracnose with heat stress, antioxidative changes and proteomic analysis in mycorrhizal cyclamen. Advances in Horticultural Science, 28(4), 195–201. https://doi.org/10.36253/ahsc-18458

Copyright (c) 2014 S. Watanabe, Y.-I. Matsubara

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Cross-protection against anthracnose with shock heat stress, antioxidative changes and proteomic analysis in mycorrhizal cyclamen were investigated. Eleven weeks after arbuscular mycorrhizal fungus (AMF, Glomus fasciculatum) inoculation, cyclamen (Cyclamen persicum, cv. Pastel) plants were acclimated under 20°C for 4 days, followed to 35°C (shock heat stress, SHS) for 7 days, and inoculated with Colletrichum gloeosporioides (CG) as anthracnose pathogen. Seven days after SHS treatment, dry weights of leaves, bulbs and roots increased in mycorrhizal plants, thus, growth promotion appeared. In addition, mycorrhizal plants showed higher resistance to anthracnose compared to non-mycorrhizal control plants. Regarding antioxidative activity, superoxide dismutase (SOD) activity increased in roots of mycorrhizal plants under 7d after SHS. DPPH radical scavenging activity increased in some parts of the mycorrhizal plants under SHS and CG-inoculated conditions. As for proteomic analysis, totally 29 spots changed in mycorrhizal plants through SHS and CG-inoculated conditions. In this case, the spot of 20.4kDa was detected only in mycorrhizal plots, furthermore, 4 spots intensively appeared in mycorrhizal plots through SHS and CG conditions. From these findings, AMF could alleviate heat shock stress with promoting host plant growth and induce resistance to anthracnose under heat stress. In addition, it supposed that antioxidative modification would have cross association with the resistance to heat shock and anthracnose, and the symbiosis-specific changes in some proteins might have concern with the cross protection.Cross-protection against anthracnose with shock heat stress, antioxidative changes and proteomic analysis in mycorrhizal cyclamen were investigated. Eleven weeks after arbuscular mycorrhizal fungus (AMF, Glomus fasciculatum) inoculation, cyclamen (Cyclamen persicum, cv. Pastel) plants were acclimated under 20°C for 4 days, followed to 35°C (shock heat stress, SHS) for 7 days, and inoculated with Colletrichum gloeosporioides (CG) as anthracnose pathogen. Seven days after SHS treatment, dry weights of leaves, bulbs and roots increased in mycorrhizal plants, thus, growth promotion appeared. In addition, mycorrhizal plants showed higher resistance to anthracnose compared to non-mycorrhizal control plants. Regarding antioxidative activity, superoxide dismutase (SOD) activity increased in roots of mycorrhizal plants under 7d after SHS. DPPH radical scavenging activity increased in some parts of the mycorrhizal plants under SHS and CG-inoculated conditions. As for proteomic analysis, totally 29 spots changed in mycorrhizal plants through SHS and CG-inoculated conditions. In this case, the spot of 20.4kDa was detected only in mycorrhizal plots, furthermore, 4 spots intensively appeared in mycorrhizal plots through SHS and CG conditions. From these findings, AMF could alleviate heat shock stress with promoting host plant growth and induce resistance to anthracnose under heat stress. In addition, it supposed that antioxidative modification would have cross association with the resistance to heat shock and anthracnose, and the symbiosis-specific changes in some proteins might have concern with the cross protection.

pdf

References

  1. ASADA K., 1999 - The water-mater cycle in chloroplasts scavening of active oxygens and dissipation of excess photons. - Ann. Rev. Plant Physiol. Plant Mol. Biol., 50: 602-639.
  2. BALL G.V., 1991 - Cyclamen (Cyclamen persicum), pp. 475-481. - In: BALL G.V. (ed.) The Ball Redbook, 15 edition. Ball Publishing, Chicago, USA.
  3. BEAUCHAMP C., FRIDOVICH I., 1971 - Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. - Anal. Biochem., 44: 276-287.
  4. BURITS M., BUCAR F., 2000 - Antioxidant activity of Nigella sativa essential oil. - Phytother. Res., 14(5): 323-328.
  5. CAMPOS-SORIANO L., GARCIA-GARRIADO J.M., SEGUNDO B.S., 2010 - Activation of basal defense mechanisms of rice plants by Glomus intraradices does not affect the arbuscular mycorrhizal symbiosis. - New Phytol., 188: 597-614.
  6. CAMPOS-SORIANO L., GARCIA-MARTINEZ J., SEGUNDO B.S., 2012 - The arbuscular mycorrhizal symbiosis promotes the systemic induction of regulatory defence-related genes in rice leaves and confers resistance to pathogen infection. - Mol. Plant Pathol., 13(6): 579-592.
  7. COMPANT S., VAN DER HEIJDEN M.G.A., SESSITSCH A., 2010 - Climate change effects on beneficial plant-microorganism interactions. - FEMS Microbial Ecol., 73: 197-214.
  8. DURAND T.C., SERGEANT K., CARPIN S., LABEL P., MORABITO D., HOUSMAN J.-F., RENAUT J., 2012 - Screening for changes in leaf and cambial proteome of Populus tremula × P. alba under different heat constraints. - J. Plant Physiol., 169: 1698-1718.
  9. ELMER W.H., MCGOVERN R.J., 2004 - Efficacy of integrating biologicals with fungicides for the suppression of Fusarium wilt of cyclamen. - Crop Prot., 23: 909-914.
  10. GARMENDIA I., AGUIRREOLEA J., GOICOECHEA N., 2006 - Defence related enzymes in pepper roots during interactions with arbuscular mycorrhizal fungi and/or Verticillium dahliae. - BioControl, 51: 293-310.
  11. GOTO T., SHIMIZU N., MORISHITA T., FUJII K., NAKANO Y., SHIMA K., 2011 - Effects of polyethylene pot removal and irrigation method on growth and flowering of gardentype cyclamen. - Acta Horticulturae, 886: 83-90.
  12. HICHEM H., MOUNIR D., NACEUR E.A., 2009 - Differencial responces of two maize (Zea mays L.) varieties to salt stress: changes on polyphenols composition of foliage and oxidative damages. - Ind. Crops Produc., 30: 144-151.
  13. ISHIZAKA H., YAMADA H., SASAKI K., 2002 - Volatile compounds in the flowers of Cyclamen persicum, C. Purpurascens and their hybrids. - Sci. Hortic., 94: 125-135.
  14. KARLSSON M.G., WERNER J.W., 2001 - Temperature after flower initiation affects morphology and flowering of cyclamen. - Sci. Hortic., 91: 357-363.
  15. KOLUPAEV YU.E., OBOZNYI A.I., SHVIDENKO N.V., 2013 - Role of hydrogen peroxide in generation of a signal inducing heat tolerance of wheat seedlings. - Russ. J. Plant Physiol., 60(2): 227-234.
  16. KUZNIAK E., SKLODOWSKA M., 2004 - The effect of Botrytis cinerea infection on the antioxidant profile of mitochondria from tomato leaves. - J. Exp. Bot., 55 (397): 605-612.
  17. LAMBAIS M.R., RIOS-RUIZ W.F., ANDRAE R.M., 2003 - Antioxidant responses in bean (Phaseoolus vulgaris) roots colonized by arbuscular mycorrhizal fungi. - New Phytol., 160(2): 421-428.
  18. LI C.-Y., ROBERTS J.K., KEY J.L., 1984 - Acquisition of thermotorelance in soybean seedlings. - Plant Physiol., 74: 152-160.
  19. LI H.Y., YANG G.D., SHU H.R., YANG Y.T., YE B.X., NISHIDA I., ZHENG C.C., 2006 - Colonization by the arbuscular mycorrhizal fungus Glomus versiforme induces a defence response against the root knot nematode Meloidogyne incognita in the grapevine (Vitis amurensis Rupr.), which includes transcriptional activation of the class III chitinase gene VCH3. - Plant Cell Physiol., 47: 154-163.
  20. LI Y., MATSUBARA Y., MIYAWAKI C., LIU Y., KOSHIKAWA K., 2008 - Temperature stress tolerance and increase in antioxidative enzyme activities in mycorrhizal strawberry plants. - Acta Horticulturae, 774: 391-395.
  21. LI Y., YANAGI A., MIYAWAKI Y., OKADA T., MATSUBARA Y., 2010 - Disease tolerance and changes in antioxidative abilities in mycorrhizal strawberry plants. - J. Japan. Soc. Hort. Sci., 79(2): 174-178.
  22. LIU J., MALDONADO-MENDOZA I., LOPEZ-MEYER M., CHEUNG F., TOWN C. D., HARRISON M.J., 2007 - Arbuscular mycorrhizal symbiosis is accompanied by local and systemic alterations in gene expression and an increase in disease resistance in the shoots. - Plant J., 50: 529-544.
  23. MARSCHNER H., DELL B., 1994 - Nutrient uptake in mycorrhizal symbiosis. - Plant Soil, 159: 89-102.
  24. MAYA M.A., MATSUBARA Y., 2013 a - Influence of arbuscular mycorrhiza on the growth and antioxidative activity in cyclamen under heat stress. - Mycorrhiza, 23: 381-390.
  25. MAYA M.A., MATSUBARA Y., 2013 b - Tolerance to fusarium wilt and anthracnose diseases and changes of antioxidative activity in mycorrhizal cyclamen. - Crop Protec., 47: 41-48.
  26. NAHIYAN A.S.M., MATSUBARA Y., 2012 - Tolerance to fusarium root rot and changes in antioxidative ability in mycorrhizal asparagus plants. - HortSci., 47: 356-360.
  27. PHILLIPS J.M., HAYMAN D.S., 1970 - Improved procedures for clearing roots and staining parasitic and VA mycorrhizal fungi for rapid assessment of infection. - Trans. Br. Mycol. Soc., 55: 158-161.
  28. ROLDAN A., DIAZ-VIANCOS P., HERNANDEZ J.A., CARRASCO L., CARAVACA F., 2008 - Superoxide dismutase and total peroxidase activities in relation to drought recovery performance of mycorrhizal shrub seedlings grown in an amended semiarid soil. - J. Plant Physiol., 165(7): 715-722.
  29. SHOKRI S., MAADI B., 2009 - Effect of arbuscular mycorrhizal fungus on the mineral nutrition and yield of Trifolium alexandrinum plants under salinity stress. - J. Agron., 8: 79-83.
  30. TERUYA R., TAKUSHI T., HIROSE N., MAKISHI Y., OOSHIRO Y., 2012 - Short hot water treatment for control of anthracnose in Mango. - Hort. Res. (Japan), 11(2): 265-271.
  31. WAHID A., GELANI S., ASHRAF M., FOOLAD M.R., 2007 - Heat tolerance in plants: An overview. - Environ. Exp. Bot., 61: 199-223.
  32. WHIPPS J.M., 2004 - Prospects and limitations for mycorrhizas in biocontrol of root pathogens. - Can. J. Bot., 82: 1198-1227.
  33. WU Q., ZOU Y., XIA R., 2006 - Effect of water stress and arbuscular mycorrhizal fungi on reactive oxygen metabolism and antioxidant production by citrus (Citrus tangerine) roots. - Eur. J. Soil Biol., 42: 166-172.
  34. YESSON C., CULHAM A., 2006 - A phyloclimatic study of cyclamen. - BMC Evol. Biol., 6: 72.
  35. YOSHINO M., WIDIASTUTI A., SONGYING Z., ODANI H., HASEGAWA M., SATO T., 2012 - Improvement of heat shock treatment and trial development of hot water sprayer for inducing disease resistance in cucumber (Cucumis sativus L.). - Hort. Res. (Japan), 11(1): 121-126.
  36. ZHU X.C., SONG F.B., LIU S.Q., LIU T.D., 2011 - Effects of arbuscular mycorrhizal fungus on photosynthesis and water status of maize under high temperature stress. - Plant Soil, 346: 189-199.