Vol. 34 No. 1 (2020): Advances in Horticultural Science
Articles

Development of interspecific hybrids between Habenaria radiata and Habenaria rhodocheila complex

P. Sinumporn
Faculty of Agriculture, Kagawa University, Miki-cho, 761-0795 Kagawa
T. Narumi-Kawasaki
Faculty of Agriculture, Kagawa University, Miki-cho, 761-0795 Kagawa
S. Fukai
Faculty of Agriculture, Kagawa University, Miki-cho, 761-0795 Kagawa

Published 2020-02-28

Keywords

  • apomixis,
  • cross combination,
  • PCR-RFLP,
  • reciprocal crossing,
  • seed germination

How to Cite

Sinumporn , P. ., Narumi-Kawasaki, T., & Fukai, S. (2020). Development of interspecific hybrids between Habenaria radiata and Habenaria rhodocheila complex. Advances in Horticultural Science, 34(1), 3–10. https://doi.org/10.13128/ahsc-8251

Abstract

Reciprocal crosses between Habenaria radiata and H. rhodocheila complex were investigated to develop new hybrids. The fruit-setting frequency and seed germination in the cross combination of H. radiata × H. rhodocheila complex were higher than those of H. rhodocheila complex × H. radiata. The hybridity of the obtained progenies was confirmed through PCR-RFLP analysis of the rRNA gene. Cross combinations producing true hybrids, apomicts, or both were observed, indicating that both H. radiata and H. rhodocheila complex were facultative apomixis. The obtained hybrids, H. radiata × H. rhodocheila (orange flower), showed the intermediate plant form and flower shape of the parents, and both petals and lip were pale yellow