Vol. 38 No. 2 (2024): Advances in Horticultural Science
Articles

Biocontrol of Fusarium spp. in vitro and in vine cuttings using Bacillus sp. F62

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  • H.C. Cavião,
  • A. Russi,
  • J. Schwambach
H.C. Cavião
Universidade de Caxias do Sul, Institute of Biotechnology, 95070-560 Caxias do Sul, Brazil.
A. Russi
Universidade de Caxias do Sul, Institute of Biotechnology, 95070-560 Caxias do Sul, Brazil.
J. Schwambach
Universidade de Caxias do Sul, Institute of Biotechnology, 95070-560 Caxias do Sul, Brazil.
DOI: https://doi.org/10.36253/ahsc-15354
Categories

Published 2024-07-01

Keywords

  • Antagonism,
  • bioagent,
  • Fusarium wilt,
  • vine rootstock

How to Cite

Cavião, H. C., Russi, A., & Schwambach, J. (2024). Biocontrol of Fusarium spp. in vitro and in vine cuttings using Bacillus sp. F62. Advances in Horticultural Science, 38(2), 189–196. https://doi.org/10.36253/ahsc-15354

Copyright (c) 2024 Helen Corso Cavião, Alessandra Russi, Joséli Schwambach

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

Fusarium spp., the causal agent of Fusarium wilt, cause substantial economic losses in viticulture, mainly in tropical regions. This study aimed to assess the biocontrol potential of Bacillus sp. F62 against Fusarium spp., both in vitro and in rootstock cuttings of the SO4 variety. To this end, the in vitro antagonism was evaluated through diffusible and volatile compounds synthesized by Bacillus sp. F62 on three Fusarium spp. isolates. Subsequently, the isolate FusA06-18 was selected for a rootstock cutting experiment. The vine cuttings underwent the following treatments: control, pathogen inoculation (Fus), bacterial inoculation (Bac), and bacterial followed by pathogen inoculation (Bac + Fus). Our findings revealed an average reduction of 39.1% in the mycelial growth of the pathogen through dual culture assay and a decrease of 11.6% in the Fusarium spp. radial growth due to the effects of volatile compounds. In the experiment with vine cuttings, applying Bacillus sp. F62 reduced the pathogen re-isolation frequency from 81.7% (Fus) to 63.3% (Bac + Fus). Therefore, Bacillus sp. F62 effectively suppressed the mycelial growth of Fusarium spp. and reduced the Fusarium wilt incidence in vine cuttings of the rootstock ‘SO4’.

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