Vol. 36 No. 3 (2022): Advances in Horticultural Science
Short note

Evaluation of Hemerocallis germplasm using single nucleotide polymorphisms of nrITS and chloroplast interspacer region

S.Y. Park
School of Biological Sciences and Technology, Chonnam National University, 500-757 Gwangju, Korea.
Y.H. Joung
School of Biological Sciences and Technology, Chonnam National University, 500-757 Gwangju, Korea.
J.K. Suh
Department of Environmental Horticulture, College of Bioresource Science, Dankook University, Cheonan, 330-714 Chungnam, Korea.
M.S. Roh
The Institute of Natural Resource Development, Mokpo National University, Muan, 58554 Jeonnam, Korea.
Published October 4, 2022
Keywords
  • Daylily,
  • haplotype,
  • nocturnal flowering,
  • polymerase chain reaction,
  • sequence analysis
How to Cite
Park, S. Y., Joung, Y. H., Suh, J. K., & Roh, M. (2022). Evaluation of Hemerocallis germplasm using single nucleotide polymorphisms of nrITS and chloroplast interspacer region. Advances in Horticultural Science, 36(3), 247-251. Retrieved from https://oaj.fupress.net/index.php/ahs/article/view/12347

Abstract

This study was initiated to distinguish nocturnal (night) flowering Hemerocallis species from day flowering species based on the single nucleotide polymorphisms (SNPs) of nuclear internal transcribed spacers 1, 2 in a ribosomal RNA gene (nrITS) and a chloroplast interspacer region (cpIS). Four nocturnal flowering species, H.  citrina, H. thunbergii, H. minor, and H. lilioasphodelus, were collected including Korea, and compared with day flowering species that included H. vespertina and H. hongdoensis. Based on the haplotypes of nrITS and cpIS, nocturnal species cannot be distinguished from day flowering species. Discrepancies in flowering time and haplotypes among H. minor accessions suggest that more germplasm with diverse geographic origins should be evaluated and identification of other genes is required to effectively distinguish nocturnal species from day flowering species.