Articles
Published 2019-01-28
Keywords
- Explant,
- In vitro,
- Glutamic Acid,
- MS medium
How to Cite
Abdi, G., Khush-hkhui, M., & Shekafandeh, A. (2019). Embryogenesis in Valerian (Valeriana officinalis L.) using leaf segments. Advances in Horticultural Science, 33(2), 179–185. https://doi.org/10.13128/ahs-24090
Abstract
Abstract:A simple procedure for direct embryogenesis without an intervening callus production was developed in valerian (Valeriana officinalis L.) using the leaf segments. Direct somatic embryogenesis were induced using by half-strength MS medium supplemented with 2,4-D (0.5 mg·l-1), glutamic acid (100 mg·l-1), 4% sucrose and 8 g·l-1 agar. Germinated embryo to form plantlets enhanced on MS medium supplemented with NAA (0.1 mg·l-1) and Kin (2 mg·l-1). Regenerated plants with well-developed root and shoot systems were successfully (72%) transferred to greenhouse.