Vol. 33 No. 2 (2019)
Articles

Embryogenesis in Valerian (Valeriana officinalis L.) using leaf segments

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  • Gholamreza Abdi,
  • Morteza Khush-hkhui,
  • Akhtar Shekafandeh
Gholamreza Abdi
Department of Biotechnology, Persian Gulf Research Institute, Persian Gulf University, Bushehr, 7516913817, Iran
Morteza Khush-hkhui
Department of Horticulture, College of Agriculture, Shiraz University, Shiraz, Iran
Akhtar Shekafandeh
Department of Horticulture, College of Agriculture, Shiraz University, Shiraz, Iran
DOI: https://doi.org/10.13128/ahs-24090

Published 2019-01-28

Keywords

  • Explant,
  • In vitro,
  • Glutamic Acid,
  • MS medium

How to Cite

Abdi, G., Khush-hkhui, M., & Shekafandeh, A. (2019). Embryogenesis in Valerian (Valeriana officinalis L.) using leaf segments. Advances in Horticultural Science, 33(2), 179–185. https://doi.org/10.13128/ahs-24090
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Abstract

Abstract:A simple procedure for direct embryogenesis without an intervening callus production was developed in valerian (Valeriana officinalis L.) using the leaf segments. Direct somatic embryogenesis were induced using by half-strength MS medium supplemented with 2,4-D (0.5 mg·l-1), glutamic acid (100 mg·l-1), 4% sucrose and 8 g·l-1 agar. Germinated embryo to form plantlets enhanced on MS medium supplemented with NAA (0.1 mg·l-1) and Kin (2 mg·l-1). Regenerated plants with well-developed root and shoot systems were successfully (72%) transferred to greenhouse.

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